Pezzella M, Pezzella F, Rapicetta M, Morace G, Macchi B, Sorice F, Baroni C D
Institute of Infectious Diseases, University of Rome La Sapienza, Italy.
Mol Cell Probes. 1989 Jun;3(2):125-32. doi: 10.1016/0890-8508(89)90023-6.
The presence of hepatitis B virus (HBV) and human immunodeficiency virus (HIV) was investigated using hybridization in 15 lymph nodes and one Kaposi's sarcoma skin lesion obtained from HIV-positive patients. Cryostat tissue sections were hybridized with chemically modified DNA probes for HBV and HIV. HIV genome was mainly observed in the cytoplasm of cells present in 7/15 lymph nodes and in the Kaposi's sarcoma skin lesion, thus indicating the expression of HIV replication. Control samples hybridized with an HTLV I probe were negative. HBV genome was found in the cytoplasm of lymphoid mononuclear cells in 2/7 lymph nodes, obtained from HIV+ patients without serum markers of ongoing HBV infection. Lymph node positivity for HBV DNA also confirms that lymphoid cells may be a target for HBV. Since HBV infection seems to precede HIV infection in nearly all patients, it is possible that it may represent a factor facilitating the development of the HIV-related disease.
利用杂交技术对15例HIV阳性患者的淋巴结和1例卡波西肉瘤皮肤病变组织进行了乙肝病毒（HBV）和人类免疫缺陷病毒（HIV）检测。用化学修饰的HBV和HIV DNA探针与恒冷箱切片组织进行杂交。HIV基因组主要在15个淋巴结中的7个以及卡波西肉瘤皮肤病变组织的细胞胞质中观察到，这表明HIV发生了复制。用HTLV I探针杂交的对照样本呈阴性。在7例无HBV感染血清标志物的HIV阳性患者的淋巴结中，有2例在淋巴单核细胞的胞质中发现了HBV基因组。淋巴结中HBV DNA阳性也证实了淋巴细胞可能是HBV的靶细胞。由于几乎所有患者中HBV感染似乎都先于HIV感染，所以HBV感染可能是促进HIV相关疾病发展的一个因素。
