Pezzella M, Pezzella F, Galli C, Macchi B, Verani P, Sorice F, Baroni C D
J Med Virol. 1987 Jun;22(2):135-42. doi: 10.1002/jmv.1890220204.
The presence of human immunodeficiency virus (HIV, or HTLV-III) genome sequence was investigated by means of in situ hybridization in cryostat sections of lymph nodes from lymphadenopathy syndrome (LAS) patients. The technique employed involved the modification of the DNA probe by chemical insertion of an antigenic sulfone group in cytosine moieties and the visualization of DNA by a double-antibody immunohistochemical reaction. The hybrid formation was revealed in five out of ten cases: in all positive samples, HIV was mainly observed in the cytoplasm of lymph node cells. The method of in situ hybridization described in the present paper is specific and has some advantages if compared with other techniques based on the use of DNA probes labelled with radioisotopes or biotin by nick translation.
通过原位杂交技术，对淋巴结病综合征（LAS）患者淋巴结冷冻切片中的人类免疫缺陷病毒（HIV，即HTLV-III）基因组序列进行了研究。所采用的技术包括通过在胞嘧啶部分化学插入抗原性砜基团来修饰DNA探针，并通过双抗体免疫组织化学反应对DNA进行可视化。在十例中有五例显示出杂交形成：在所有阳性样本中，HIV主要在淋巴结细胞的细胞质中观察到。与其他基于用放射性同位素或生物素通过缺口平移标记DNA探针的技术相比，本文所述的原位杂交方法具有特异性且有一些优点。
