Dehghan Morteza, Pourahmad-Jaktaji Razieh, Farzaneh Zarghampoor
Department of Orthopedics, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Genetics, University of Shahrekord, Shahrekord, Iran.
Acta Inform Med. 2016 Jul 16;24(4):239-243. doi: 10.5455/aim.2016.24.239-243.
Calcitonin receptor gene has also a polymorphism which is associated with bone mass density. This study evaluates the association between calcitonin receptor AluI (rs1801197) and Taq1 calcitonin genes polymorphism with bone density rate.
In this descriptive-analytical study in 2013 in southwestern Iran, 200 blood samples, per the Cochran sample size formula, were taken from women aged 45 and older. DNA was extracted from the samples using the phenol- chloroform method and the genomic fragments in question were proliferated using the polymerase chain reaction (PCR) method.
The genotypic distribution of polymorphism AluI for , , and genotypes in control group was 31.4%, 38.6%, and 30% and in patients 25.4%, 55.4%, and 19.2%, respectively. There was no significant difference in polymorphism AluI between patients and control group and no significant association was found between this gene and bone density rate (P > 0.05). All patients and the individuals in the control group exhibited genotype for TaqI calcitonin gene and no significant association was found between these participants and osteoporosis.
There was no association between two polymorphisms and osteoporosis, and between polymorphism of these two genes and osteoporosis development rate in the participants.
降钙素受体基因也存在一种与骨密度相关的多态性。本研究评估降钙素受体AluI（rs1801197）和Taq1降钙素基因多态性与骨密度率之间的关联。
在2013年于伊朗西南部进行的这项描述性分析研究中，根据 Cochr an样本量公式，从45岁及以上女性中采集了200份血样。使用酚 - 氯仿法从样本中提取DNA，并采用聚合酶链反应（PCR）法扩增相关基因组片段。
对照组中，AluI多态性的基因型分布为，，和基因型分别为31.4%、38.6%和30%，患者组中分别为25.4%、55.4%和19.2%。患者组与对照组之间AluI多态性无显著差异，该基因与骨密度率之间也未发现显著关联（P>0.05）。所有患者和对照组个体均表现出TaqI降钙素基因的基因型，这些参与者与骨质疏松症之间未发现显著关联。
这两种多态性与骨质疏松症之间，以及这两个基因的多态性与参与者骨质疏松症发生率之间均无关联。
