Cherepanov S A, Cherepanova K I, Grinenko N F, Antonova O M, Chekhonin V P
N. I. Pirogov Russian National Research Medical University, Moscow, Russia.
V. P. Serbsky Federal Medical Research Center of Psychiatry and Narcology, Moscow, Russia.
Bull Exp Biol Med. 2016 Sep;161(5):674-678. doi: 10.1007/s10517-016-3483-2. Epub 2016 Oct 5.
We studied the effect of an activator (ShTh) and an inhibitor (cyclopamine) of the Hedgehog signaling pathway on proliferation of human glioma cell lines U87-MG and U251-MG and cultured human astrocytes. The Hedgehog signaling pathway is activated in glioma cells, but not in cultured human astrocytes. Experiments with Shh and cyclopamine can serve as an additional criterion for assessing activity of Hedgehog signaling in known cell lines and primary cultured cells.
我们研究了刺猬信号通路的激活剂（ShTh）和抑制剂（环杷明）对人胶质瘤细胞系U87-MG和U251-MG以及培养的人星形胶质细胞增殖的影响。刺猬信号通路在胶质瘤细胞中被激活，但在培养的人星形胶质细胞中未被激活。使用Shh和环杷明进行的实验可作为评估已知细胞系和原代培养细胞中刺猬信号活性的额外标准。
