Zhang Jie, Zhang Haihao, Zhao Yilin, Guo Chen, Yang Zhaoqing, Ma Shaohui
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College (CAMS and PUMC), 935 Jiao Ling Road, Kunming, 650118, Yunnan Province, China.
Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Kunming, 650118, China.
Arch Virol. 2017 Jan;162(1):307-311. doi: 10.1007/s00705-016-3075-5. Epub 2016 Oct 1.
Human coxsackievirus B2 (CVB2) belongs to the species Human enterovirus B and can cause aseptic meningitis, myocarditis and hand-foot-mouth disease (HFMD). We first determined the complete genome of the RW41-2/YN/CHN/2012 strain, isolated from a patient with HFMD and aseptic meningitis in the Yunnan Province, China in 2012. The strain shared 83.5 % and 82.2 % nucleotide similarity with CVB2 prototype strain Ohio-1, in the complete VP1 gene and the complete genome, respectively. Using phylogenetic and homogeneity analyses for the complete VP1 gene, CVB2 strains could be divided into four genogroups (A-D); the RW41-2/YN/CHN/2012 strain belonging to genogroup D. The amino acid sequence of VP1 is highly conserved. Recombination analyses showed the newly isolated RW41-2/YN/CHN/2012 strain was probably a recombinant, which was closely related to strain CVB2 (KM386639) in the genomic P1 and P2 regions and strains of other human enterovirus B (HEV-B) viruses (KT353721, JX644073, and KP262053) in the P3 region.
人柯萨奇病毒B2（CVB2）属于人肠道病毒B种，可引起无菌性脑膜炎、心肌炎和手足口病（HFMD）。我们首次测定了2012年从中国云南省一名手足口病和无菌性脑膜炎患者分离出的RW41-2/YN/CHN/2012株的全基因组。该毒株在完整的VP1基因和全基因组中，与CVB2原型株Ohio-1的核苷酸相似性分别为83.5%和82.2%。通过对完整VP1基因进行系统发育和同源性分析，CVB2毒株可分为四个基因群（A-D）；RW41-2/YN/CHN/2012株属于基因群D。VP1的氨基酸序列高度保守。重组分析表明，新分离的RW41-2/YN/CHN/2012株可能是一个重组体，在基因组P1和P2区域与CVB2毒株（KM386639）密切相关，在P3区域与其他人肠道病毒B（HEV-B）病毒株（KT353721、JX644073和KP262053）密切相关。
