菲律宾犬类感染沃氏巴贝斯虫的首例报告。
First report on Babesia vogeli infection in dogs in the Philippines.
作者信息
Ybañez Adrian P, Ybañez Rochelle Haidee D, Talle MaxFrancis G, Liu Mingming, Moumouni Paul Franck Adjou, Xuan Xuenan
机构信息
Biology and Environmental Studies Program, Sciences Cluster, University of the Philippines Cebu, Lahug, Cebu City, Philippines; Gullas College of Medicine, University of the Visayas, Banilad, Cebu City 6000, Philippines.
Biology and Environmental Studies Program, Sciences Cluster, University of the Philippines Cebu, Lahug, Cebu City, Philippines.
出版信息
Parasitol Int. 2017 Feb;66(1):813-815. doi: 10.1016/j.parint.2016.10.001. Epub 2016 Oct 3.
Babesia vogeli is a tick-borne protozoal pathogen that infects erythrocytes. In Southeast Asia, this pathogen has only been reported in Thailand. In this study, nine dogs presented at three different veterinary clinics in Cebu City, Philippines were found positive for B. vogeli. DNA was extracted from blood samples and tested using a PCR for genus Babesia and a PCR specific for B. vogeli (both based on the 18S rRNA gene). Blood smears (triplicate) from each sample were found negative. All positive amplicons were sequenced and were found to be 99.4% identical to registered B. vogeli sequences at Genbank. Phylogenetic analysis revealed monophyletic grouping of Philippine sequences with the registered A. platys Genbank sequences. This is the first report of B. vogeli infection in dogs in the Philippines.
伯氏沃热梨浆虫是一种通过蜱传播的原生动物病原体，可感染红细胞。在东南亚，这种病原体仅在泰国被报道过。在本研究中，菲律宾宿务市三家不同兽医诊所收治的9只犬被检测出伯氏沃热梨浆虫呈阳性。从血样中提取DNA，并使用针对巴贝斯属的聚合酶链反应（PCR）和针对伯氏沃热梨浆虫的特异性PCR（均基于18S rRNA基因）进行检测。每个样本的血涂片（一式三份）均呈阴性。所有阳性扩增子均进行了测序，发现与Genbank中注册的伯氏沃热梨浆虫序列有99.4%的同一性。系统发育分析显示，菲律宾序列与Genbank中注册的犬血小板无形体序列形成单系群。这是菲律宾犬感染伯氏沃热梨浆虫的首次报道。
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