Ybañez Rochelle Haidee D, Ybañez Adrian P, Arnado Lyra Lee A, Belarmino Laila Monika P, Malingin Knowlie Gay F, Cabilete Paul Bien C, Amores Ziggy Ryan O, Talle Maxfrancis G, Liu Mingming, Xuan Xuenan
College of Science, University of the Philippines Cebu, Gorordo Avenue, Lahug, Cebu City 6000, Philippines.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro City 080-8555, Japan.
Vet World. 2018 Jan;11(1):14-19. doi: 10.14202/vetworld.2018.14-19. Epub 2018 Jan 12.
, , and spp. are canine pathogens transmitted by the tick which can cause varied clinical signs. These pathogens have been investigated in the Philippines, but coinfection has not been reported yet.
The aim of this study was to evaluate the presence of and spp. in Philippine dogs.
A total of 100 dogs from seven different veterinary establishments in Cebu, Philippines, were examined for and spp. infection using peripheral blood smear examination and polymerase chain reaction (PCR). Inclusion criteria included a history or presence of tick infestation, anemia, and/or thrombocytopenia. Clinical signs were recorded. Statistical analyses were performed between PCR positivity and clinical signs and hematological results.
A total of 10 and 18 dogs were found to be positive for and spp., respectively. One animal was PCR positive for both pathogens, which is the first report of coinfection in the country. The most common clinical signs observed include inappetence (89%), lethargy (80%), thrombocytopenia (85%), and anemia (74%). Analyses revealed that inappetence (p=0.044) and weight loss (p=0.028) were found statistically significant with infection. Basophil (p=0.001) and eosinophil counts (p=0.000) were also found significantly different between spp.-positive and -negative dogs. On the other hand, differential monocyte count (p=0.009) was found significantly different between spp.-positive and -negative dogs.
The present study showed low infection rates of canine ehrlichiosis/anaplasmosis and babesiosis and provided additional evidence for the presence of the pathogens in the area.
埃立克体属、无形体属和巴贝斯虫属的物种是由蜱传播的犬类病原体，可导致多种临床症状。这些病原体已在菲律宾进行过调查，但尚未有共感染的报道。
本研究的目的是评估菲律宾犬只中埃立克体属和无形体属物种以及巴贝斯虫属的存在情况。
对来自菲律宾宿务市七家不同兽医机构的总共100只犬只进行了检查，采用外周血涂片检查和聚合酶链反应（PCR）检测埃立克体属、无形体属物种以及巴贝斯虫属感染情况。纳入标准包括有蜱虫寄生史或存在蜱虫寄生、贫血和/或血小板减少症。记录临床症状。对PCR阳性结果与临床症状及血液学结果进行统计学分析。
分别有10只和18只犬只被发现埃立克体属和无形体属物种以及巴贝斯虫属呈阳性。有一只动物两种病原体的PCR检测均呈阳性，这是该国共感染的首次报道。观察到的最常见临床症状包括食欲不振（89%）、嗜睡（80%）、血小板减少症（85%）和贫血（74%）。分析显示，食欲不振（p=0.044）和体重减轻（p=0.028）与埃立克体属感染在统计学上具有显著相关性。嗜碱性粒细胞（p=0.001）和嗜酸性粒细胞计数（p=0.000）在无形体属物种阳性和阴性犬只之间也存在显著差异。另一方面，单核细胞分类计数在无形体属物种阳性和阴性犬只之间存在显著差异（p=0.009）。
本研究显示犬埃立克体病/无形体病和巴贝斯虫病的感染率较低，并为该地区存在这些病原体提供了更多证据。
