Azmi Kifaya, Al-Jawabreh Amer, Nasereddin Abedelmajeed, Abdelkader Ahmad, Zaid Taher, Ereqat Suheir, Sawalha Samer S, Baneth Gad, Abdeen Ziad
Biochemistry and Molecular Biology Department,Faculty of Medicine,Al-Quds University,Abu Deis,The West Bank,Palestine.
Al-Quds Public Health Society,Jerusalem,Palestine.
Parasitology. 2017 Apr;144(5):613-621. doi: 10.1017/S0031182016002201. Epub 2016 Dec 12.
Dogs serve as hosts for a great number of parasites, which may affect their health and wellbeing. This study aimed to observe tick borne pathogens in dogs from Palestine including Hepatozoon canis and Babesia species. The prevalence of both H. canis and Babesia species infections in apparently healthy dogs, from ten districts of the West Bank was surveyed. DNA was extracted from blood samples obtained from dogs (n = 362) and ticks (n = 213) collected from dogs (n = 77). A primer set that amplifies a partial sequence of the Babesia and Hepatozoon 18S rRNA gene was used for PCR and the DNA sequences of the PCR products of all samples were determined. Twenty-nine (8·0%) of the dogs were found infected including 20 with H. canis (5·5%), seven with Babesia vogeli (1·9%) and two with undefined Babesia spp. (0·6%). Twelve Rhipicephalus sanguineus s.l ticks were pathogen-positive, including ten with H. canis (4·7%), one with B. vogeli (0·5%), and one with Hepatozoon felis (0·5%). The results indicated that a wide range of tick borne pathogens is circulating in the canine population in the surveyed region. This study is the first report on the prevalence of H. canis, B. vogeli and Babesia spp. in dogs in Palestine and its results will assist in the management of diseases associated with these blood parasites.
狗是大量寄生虫的宿主，这些寄生虫可能会影响它们的健康和福祉。本研究旨在观察巴勒斯坦犬只身上的蜱传病原体，包括犬肝簇虫和巴贝斯虫属。对来自约旦河西岸十个地区看似健康的犬只中犬肝簇虫和巴贝斯虫属感染的患病率进行了调查。从362只犬只采集的血液样本以及从77只犬只身上采集的213只蜱虫中提取DNA。使用一组能扩增巴贝斯虫和肝簇虫18S rRNA基因部分序列的引物进行聚合酶链反应（PCR），并测定所有样本PCR产物的DNA序列。发现29只（8.0%）犬只受到感染，其中20只感染犬肝簇虫（5.5%），7只感染韦氏巴贝斯虫（1.9%），2只感染未明确的巴贝斯虫属（0.6%）。12只血红扇头蜱（狭义）呈病原体阳性，其中10只感染犬肝簇虫（4.7%），1只感染韦氏巴贝斯虫（0.5%），1只感染猫肝簇虫（0.5%）。结果表明，在所调查地区的犬类群体中，多种蜱传病原体在传播。本研究是关于巴勒斯坦犬只中犬肝簇虫、韦氏巴贝斯虫和巴贝斯虫属患病率的首次报告，其结果将有助于管理与这些血液寄生虫相关的疾病。
