Department of Mechanical Engineering, Sogang University, Seoul, Korea.
Microfluidics group, KIST-Europe, Saarbrücken, Germany and Mechanotronics Department, Universität des Saarlandes, Saarbrücken, Germany.
Lab Chip. 2016 Oct 5;16(20):3866-3884. doi: 10.1039/c6lc00984k.
The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules across several orders of magnitude. Since the first application of PCR in a microfluidic device was developed in 1998, an increasing number of researchers have continued the development of microfluidic PCR systems. In this review, we introduce recent developments in microfluidic-based space and time domain devices as well as discuss various designs integrated with multiple functions for sample preparation and detection. The development of isothermal nucleic acid amplification and digital PCR microfluidic devices within the last five years is also highlighted. Furthermore, we introduce various commercial microfluidic PCR devices.
聚合酶链反应（PCR）的发明引发了分子生物学的革命，为扩增脱氧核糖核酸（DNA）分子提供了一种方法，可以跨越几个数量级进行扩增。自 1998 年首次在微流控装置中应用 PCR 以来，越来越多的研究人员继续开发微流控 PCR 系统。在这篇综述中，我们介绍了基于微流控的时-空域装置的最新进展，并讨论了各种集成了多种功能的设计，用于样品制备和检测。还强调了过去五年内等温核酸扩增和数字 PCR 微流控装置的发展。此外，我们还介绍了各种商业微流控 PCR 设备。
