Cheng Ji, Shuai Xiaoming, Gao Jinbo, Cai Ming, Wang Guobin, Tao Kaixiong
Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Oncotarget. 2016 Nov 15;7(46):75739-75748. doi: 10.18632/oncotarget.12405.
AMPK is a well-investigated kinase mediating cellular metabolism and stress responses. However, its indicative role in survival prognosis remains ill-defined. Therefore we performed this meta-analysis in order to clarify the prognostic impact of AMPK expression in human malignancies.
Literatures were retrieved via searching databases of PubMed, Web of Science, Embase and Cochrane Library. Studies comparing the prognostic significance between different AMPK levels among human malignancies were included into the pooled analysis. The statistical procedures were conducted by Review Manager 5.3 and the effect size was displayed by model of odds ratio. Subgroup analyses were additionally implemented to disclose the potential confounding elements. The outcome stability was examined by sensitivity analysis, and both Begg's test and Egger's test were utilized to detect the publication bias across the included studies.
21 retrospective cohorts were eventually obtained with a total sample-size of 9987 participants. Patients with higher AMPK expression had better outcomes of 3-year overall survival (P<0.0001), 5-year overall survival (P<0.0001), 10-year overall survival (P<0.0001), 3-year disease free survival (P<0.0001), 5-year disease free survival (P=0.002) and 10-year disease free survival (P=0.0004). Moreover, the majority of subgroup results also verified the favorably prognostic significance of AMPK over-expression. The outcome stability was confirmed by sensitivity analysis. Results of Begg's (P=0.76) and Egger's test (P=0.09) suggested that there was no publication bias within the included trials.
Higher expression of AMPK significantly indicates better prognosis in human malignancies.
AMPK是一种经过充分研究的激酶，可介导细胞代谢和应激反应。然而，其在生存预后中的指示作用仍不明确。因此，我们进行了这项荟萃分析，以阐明AMPK表达在人类恶性肿瘤中的预后影响。
通过检索PubMed、Web of Science、Embase和Cochrane图书馆数据库获取文献。纳入比较人类恶性肿瘤中不同AMPK水平预后意义的研究进行汇总分析。采用Review Manager 5.3进行统计分析，效应量以比值比模型表示。另外进行亚组分析以揭示潜在的混杂因素。通过敏感性分析检验结果的稳定性，并利用Begg检验和Egger检验检测纳入研究中的发表偏倚。
最终获得21个回顾性队列，总样本量为9987名参与者。AMPK表达较高的患者在3年总生存期（P<0.0001）、5年总生存期（P<0.0001）、10年总生存期（P<0.0001）、3年无病生存期（P<0.0001）、5年无病生存期（P=0.002）和10年无病生存期（P=0.0004）方面有更好的预后。此外，大多数亚组结果也证实了AMPK过表达具有良好的预后意义。敏感性分析证实了结果的稳定性。Begg检验（P=0.76）和Egger检验（P=0.09）结果表明纳入的试验中不存在发表偏倚。
AMPK的高表达显著表明人类恶性肿瘤的预后较好。
