胚胎植入期间小鼠子宫中ATF6α的表达与调控

Expression and regulation of ATF6α in the mouse uterus during embryo implantation.

作者信息

Xiong Yongjie, Li Wenzhe, Lin Pengfei, Wang Lei, Wang Nan, Chen Fenglei, Li Xiao, Wang Aihua, Jin Yaping

机构信息

Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China.

College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.

出版信息

Reprod Biol Endocrinol. 2016 Oct 7;14(1):65. doi: 10.1186/s12958-016-0199-0.

DOI:10.1186/s12958-016-0199-0

PMID:27717400

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5055674/

Abstract

BACKGROUND

ATF6α, one of the sensor proteins in the stress signaling pathway of the endoplasmic reticulum, is located in the membrane of the endoplasmic reticulum. To date, the physiological function of ATF6α in the process of embryo implantation has not been reported.

METHODS

In this study, the expression pattern of ATF6α in the mouse uterus during peri-implantation and the estrous cycle was detected by real-time PCR, western blot and immunohistochemistry.

RESULTS

ATF6α mRNA and protein levels were higher in the uterus near the implantation site on day 5 and were intensely expressed in the secondary decidual zone (SDZ) on days 7-8. In the uteri of pseudopregnant mice, ATF6α mRNA and protein levels were lower on day 5 than on other days. The activating blastocyst and artificial decidualization had an obvious effect of increasing the expression of ATF6α. In addition, the expression of ATF6α was affected by progesterone (P) and estrogen (E) in ovariectomized mice. This finding is further supported by evidence from mice during the estrous cycle.

CONCLUSIONS

Thus, we have concluded that ATF6α may play an important role during embryo implantation and decidualization.

摘要

背景

活化转录因子6α（ATF6α）是内质网应激信号通路中的传感蛋白之一，位于内质网膜上。迄今为止，尚未见ATF6α在胚胎着床过程中的生理功能报道。

方法

本研究采用实时荧光定量PCR、蛋白质免疫印迹法及免疫组织化学法，检测小鼠围着床期及发情周期子宫中ATF6α的表达模式。

结果

第5天着床部位附近子宫中ATF6α的mRNA和蛋白水平较高，第7 - 8天在次级蜕膜区（SDZ）有强烈表达。在假孕小鼠子宫中，第5天ATF6α的mRNA和蛋白水平低于其他天数。活化囊胚和人工蜕膜化对ATF6α表达有明显的上调作用。此外，去卵巢小鼠中ATF6α的表达受孕酮（P）和雌激素（E）影响。发情周期小鼠的证据进一步支持了这一发现。