Mosca Fabio, Madeddu Manuela, Sayed Ahmad Abdel, Zaniboni Luisa, Iaffaldano Nicolaia, Cerolini Silvia
Department of Veterinary Medicine, University of Milan, via Trentacoste 2, 20134, Milan, Italy.
Department of Veterinary Medicine, University of Milan, via Trentacoste 2, 20134, Milan, Italy.
Cryobiology. 2016 Dec;73(3):343-347. doi: 10.1016/j.cryobiol.2016.10.001. Epub 2016 Oct 4.
The aim of this study was to assess the combined effect of dimethylacetamide (DMA) and two non-permeating cryoprotective agents, trehalose and sucrose, on the quality of post-thaw chicken semen. Adult Lohmann roosters (n = 27) were used. Semen was processed according to the following treatments: Lake pre-freezing extender +6% DMA (LPF, control treatment), LPF + 0.1 M trehalose (LPF-T treatment), LPF + 0.1 M sucrose (LPF-S treatment) and LPF + 0.1 M trehalose + 0.1 M sucrose (LPF-TS treatment). Semen was loaded into straws and frozen in nitrogen vapour. Sperm quality (viability, mobility and kinetic parameters) was assessed immediately after thawing (T0) and at 5 (T5), 10 (T10) and 15 min (T15) thereafter. The different cryodiluent combinations significantly affected the kinetic parameters. The presence of trehalose, alone or with sucrose, combined with DMA improved the quality of motion in cryopreserved sperm in comparison to DMA alone (LPF) and DMA with sucrose (LPF-S). In particular, the highest values in linearity (LIN) and wobble (WOB) were measured in the treatment LPF-T. The treatments significantly affected the recovery rate of progressive motile sperm that presented the best value soon after thawing in the LPF-T treatment; moreover, the presence of trehalose, alone (LPF-T) or with sucrose (LPF-TS), significantly improved the recovery rate of progressive motile sperm also at T5 and T10 compared to LPF and LPF-S. The present results show a positive synergic action of DMA and trehalose on motile function of thawed chicken sperm.
本研究的目的是评估二甲基乙酰胺（DMA）与两种非渗透性冷冻保护剂海藻糖和蔗糖对解冻后鸡精液质量的联合作用。使用成年罗曼公鸡（n = 27）。精液根据以下处理方法进行处理：莱克预冻稀释液+6% DMA（LPF，对照处理）、LPF + 0.1 M海藻糖（LPF-T处理）、LPF + 0.1 M蔗糖（LPF-S处理）以及LPF + 0.1 M海藻糖+ 0.1 M蔗糖（LPF-TS处理）。精液装入细管并在氮蒸气中冷冻。解冻后立即（T0）以及此后5分钟（T5）、10分钟（T10）和15分钟（T15）评估精子质量（活力、运动性和动力学参数）。不同的冷冻稀释剂组合显著影响动力学参数。与单独使用DMA（LPF）和DMA与蔗糖（LPF-S）相比，海藻糖单独或与蔗糖一起与DMA联合使用可改善冷冻保存精子的运动质量。特别是，在LPF-T处理中测得线性度（LIN）和摆动度（WOB）的最高值。这些处理显著影响了渐进性活动精子的回收率，在LPF-T处理中解冻后不久该值最佳；此外，与LPF和LPF-S相比，海藻糖单独（LPF-T）或与蔗糖一起（LPF-TS）在T5和T10时也显著提高了渐进性活动精子的回收率。目前的结果表明DMA和海藻糖对解冻后鸡精子的运动功能具有积极的协同作用。
