Nakagomi T, Do L P, Agbemabiese C A, Kaneko M, Gauchan P, Doan Y H, Jere K C, Steele A D, Iturriza-Gomara M, Nakagomi O, Cunliffe N A
Department of Hygiene and Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK.
Arch Virol. 2017 Jan;162(1):213-226. doi: 10.1007/s00705-016-3103-5. Epub 2016 Oct 7.
Rotavirus A strains detected in diarrhoeal children commonly possess any one of the genotypes G1, G2, G3, G4, and G9, with a recent increase in G12 detection globally. G12P[6] strains possessing short RNA (DS-1-like) and long RNA (Wa-like) migration patterns accounted for 27 % of the strains circulating in Blantyre, Malawi, between 2007 and 2008. To understand how the G12P[6] strains with two distinct genetic backgrounds emerged in Malawi, we conducted whole-genome analysis of two long-RNA and two short-RNA strains. While the former had a typical Wa-like genotype constellation of G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1, the latter was found to have G12-P[6]-I2-R2-C2-M1-A2-N2-T2-E2-H2: a VP3 gene mono-reassortant on the DS-1-like backbone. Phylogenetic and Bayesian Markov chain Monte Carlo analyses showed that the short-RNA G12P[6] strains were generated around 2006 by reassortment between an African Wa-like G12P[6] strain donating three genes (the VP7, VP4, and VP3 genes) and a G2P[4] strain similar to the one circulating in Thailand or the United States of America that donated the remaining eight genes. On the other hand, the long-RNA strains were generated as a result of reassortment events within Wa-like G12 and non-G12 strains commonly circulating in Africa; only the VP4 gene was from a Malawian G8P[6] strain. In conclusion, this study uncovered the evolutionary pathways through which two distinct G12P[6] strains emerged in Malawi.
在腹泻儿童中检测到的A组轮状病毒毒株通常具有G1、G2、G3、G4和G9基因型中的任何一种，最近全球范围内G12基因型的检测有所增加。2007年至2008年间，具有短RNA（DS-1样）和长RNA（Wa样）迁移模式的G12P[6]毒株占马拉维布兰太尔流行毒株的27%。为了解具有两种不同遗传背景的G12P[6]毒株如何在马拉维出现，我们对两株长RNA和两株短RNA毒株进行了全基因组分析。前者具有典型的Wa样基因型组合G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1，而后者被发现具有G12-P[6]-I2-R2-C2-M1-A2-N2-T2-E2-H2：一种基于DS-1样主干的VP3基因单重配毒株。系统发育和贝叶斯马尔可夫链蒙特卡罗分析表明，短RNA G12P[6]毒株是在2006年左右由一株捐赠三个基因（VP7、VP4和VP3基因）的非洲Wa样G12P[6]毒株与一株类似于在泰国或美国流行的捐赠其余八个基因的G2P[4]毒株之间重配产生的。另一方面，长RNA毒株是非洲常见的Wa样G12和非G12毒株之间重配事件的结果；只有VP4基因来自马拉维G8P[6]毒株。总之，本研究揭示了两种不同的G12P[6]毒株在马拉维出现的进化途径。
