Theamboonlers A, Maiklang O, Thongmee T, Chieochansin T, Vuthitanachot V, Poovorawan Y
Department of Pediatrics, Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Chum Phae Hospital, Chum Phae, Khon Kaen, Thailand.
Infect Genet Evol. 2014 Jan;21:295-302. doi: 10.1016/j.meegid.2013.11.020. Epub 2013 Dec 1.
This study has identified diverse and re-assorted group A rotavirus (RVA) strains by sequence and phylogenetic analysis of the 11 genomic segments. The 22 cases investigated in this study were collected from children with diarrhea between 2008 and 2011. The RVA genomic constellations identified in this study were identified as G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 22.7% (5/22); G2-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2 27.3% (6/22); G3-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 18.2% (4/22); G3-P[9]-I3-R3-C3-M3-A3-N3-T3-E3-H6 4.6% (1/22); G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 9.1% (2/22); G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 4.6% (1/22) and G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 13.6% (3/22). Two RVA strains, possessing a complete AU-1-like genomic backbone, showed re-assortment for genes 3 and 11, revealing possible zoonotic re-assortment events between human and canine strains. In addition, one of the analyzed strains revealed a G12 specificity for VP7 in combination with a porcine-like P[6] VP4 and a complete Wa-like constellation. Continuous surveillance of rotavirus strains and their evolution may be useful for understanding the emergence of novel strains through interspecies genome re-assortment between human and animal viruses.
本研究通过对11个基因组片段进行序列和系统发育分析，鉴定出了多种重配的A组轮状病毒（RVA）毒株。本研究调查的22例病例收集于2008年至2011年期间腹泻儿童。本研究中鉴定出的RVA基因组组合为：G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1，占22.7%（5/22）；G2-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2，占27.3%（6/22）；G3-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1，占18.2%（4/22）；G3-P[9]-I3-R3-C3-M3-A3-N3-T3-E3-H6，占4.6%（1/22）；G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1，占9.1%（2/22）；G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1，占4.6%（1/22）；G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1，占13.6%（3/22）。两株RVA毒株具有完整的AU-1样基因组骨架，在基因3和11处出现重配，揭示了人与犬毒株之间可能存在的人畜共患病重配事件。此外，其中一株分析毒株显示VP7具有G12特异性，同时伴有猪样P[6] VP4和完整的Wa样组合。持续监测轮状病毒毒株及其进化情况，可能有助于了解通过人类和动物病毒之间的种间基因组重配产生新型毒株的情况。
