Guo Xinrong, Wu Fangying, Ni Yongnian, Kokot Serge
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China; School of Chemistry, Nanchang University, Nanchang, 330031, China.
School of Chemistry, Nanchang University, Nanchang, 330031, China.
Anal Chim Acta. 2016 Oct 26;942:112-120. doi: 10.1016/j.aca.2016.08.047. Epub 2016 Sep 9.
A strong red fluorescent nanocomposite, consisting of graphite-like carbon nitride nanosheets (g-CN NSs) and serum albumin-capped Au nanoclusters (AuNCs), was synthesized. Dopamine (DA) can quench the red fluorescence of the nanocomposite, based on the Forster resonance energy transfer (FRET) mechanism. In this quenching process, the energy is transferred from the fluorescent g-CN NSs-AuNCs to the oxidized DA quinine molecules (DA is easily oxidated to form DA quinine in air). The red fluorescence emission at 420 nm decreases dramatically and the quenching ratio (F - F)/F is linearly related to the concentration of DA in the range of 0.05-8.0 μmol L with a detection limit of 0.018 μmol L (S/N = 3). Additionally, this sensor has a potential of application to assay the DA in the real samples, such as human serum and human urine.
合成了一种由类石墨氮化碳纳米片（g-CN NSs）和血清白蛋白包覆的金纳米簇（AuNCs）组成的强红色荧光纳米复合材料。基于福斯特共振能量转移（FRET）机制，多巴胺（DA）可以淬灭该纳米复合材料的红色荧光。在这个淬灭过程中，能量从荧光性的g-CN NSs-AuNCs转移到氧化的多巴胺醌分子（DA在空气中容易氧化形成多巴胺醌）。420 nm处的红色荧光发射显著降低，淬灭率（F₀ - F）/F₀与0.05 - 8.0 μmol L⁻¹范围内的DA浓度呈线性关系，检测限为0.018 μmol L⁻¹（S/N = 3）。此外，该传感器具有应用于检测实际样品（如人血清和人尿液）中DA的潜力。
