通过金纳米团簇和聚合物保护的金纳米粒子组合实现用于L-半胱氨酸开启式传感的荧光探针。
Fluorescent probe for turn-on sensing of L-cysteine by ensemble of AuNCs and polymer protected AuNPs.
作者信息
Xu Xiaozhe, Qiao Juan, Li Nan, Qi Li, Zhang Shufeng
机构信息
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing 100190, PR China; College of Chemistry, Tianjin Normal University, No. 393, Extension of Bin Shui West Road, Xiqing District, Tianjin 300387, PR China.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing 100190, PR China.
出版信息
Anal Chim Acta. 2015 Jun 16;879:97-103. doi: 10.1016/j.aca.2015.03.036. Epub 2015 Mar 26.
A new fluorescent probe based on ensemble of gold nanoclusters (AuNCs) and polymer protected gold nanoparticles (AuNPs) for turn-on sensing of L-cysteine was designed and prepared. The AuNCs were protected by bovine serum albumin and had strong fluorescence. The polymer protected AuNPs were synthesized by a facile in situ strategy at room temperature and could quench the fluorescence of AuNCs due to the Förster resonance energy transfer. Interestingly, it has been observed that the quenched fluorescence of AuNCs was recovered by L-cysteine, which could induce the aggregation of polymer protected AuNPs by sulfur group. Then the prepared fluorescent probe was successfully used for determination of L-Cys in human urines, which would have an evolving aspect and promote the subsequent exploration.
设计并制备了一种基于金纳米簇（AuNCs）和聚合物保护的金纳米颗粒（AuNPs）组合的新型荧光探针，用于L-半胱氨酸的开启式传感。AuNCs由牛血清白蛋白保护，具有很强的荧光。聚合物保护的AuNPs在室温下通过简便的原位策略合成，由于Förster共振能量转移可猝灭AuNCs的荧光。有趣的是，已观察到L-半胱氨酸可恢复AuNCs猝灭的荧光，L-半胱氨酸可通过硫基团诱导聚合物保护的AuNPs聚集。然后，所制备的荧光探针成功用于测定人尿液中的L-半胱氨酸，这将具有不断发展的前景并促进后续探索。
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