Sridhar R, Koch C J, Stroude E C, Inch W R
Br J Cancer Suppl. 1978 Jun;3:141-4.
We found that dehydroascorbic acid (DHA), 5-thio-D-glucose (5TG) and 2-deoxy-D-glucose (2DG) were toxic to monolayers of V79 cells under hypoxic conditions at 37 degree C but not at 0 degree C. Very little toxicity was observed at either temperature under aerobic conditions. When spheroids (600 micrometer diameter) were treated with 7.5 mM of these chemicals for periods up to 3 days, the number of viable cells per spheroid dropped to 70% for DHA, 50% for 5TG and 25% for 2DG relative to controls. These survivals were reduced to much lower values when the experiments were repeated in glucose-free medium.
我们发现，脱氢抗坏血酸（DHA）、5-硫代-D-葡萄糖（5TG）和2-脱氧-D-葡萄糖（2DG）在37℃缺氧条件下对V79细胞单层有毒性，但在0℃时无毒性。在有氧条件下，两种温度下均观察到极低的毒性。当用7.5 mM这些化学物质处理直径600微米的球体长达3天时，相对于对照组，每个球体的活细胞数量对于DHA降至70%，对于5TG降至50%，对于2DG降至25%。当在无葡萄糖培养基中重复实验时，这些存活率降低到更低的值。
