Abdulla Riaz, Tellis Rouchelle Charmaine, Athikari Roshan, Kudkuli Jagadish
Department of Oral Pathology and Microbiology, Yenepoya Dental College, Yenepoya University, Deralakatte, Mangalore, Karnataka, India.
Department of Microbiology, Yenepoya Medical College, Yenepoya University, Deralakatte, Mangalore, Karnataka, India.
J Oral Maxillofac Pathol. 2016 Sep-Dec;20(3):390-394. doi: 10.4103/0973-029X.190910.
Nonsyndromic cleft lip with or without palate (NSCL ± P) is a genetic predisposition involving defects in shape and makeup of the lip and palate. Elevation of homocysteine (Hcy) levels is seen in medical complications such as developmental anomalies causing neural tube defects, congenital vascular diseases, neurodegenerative and psychiatric conditions. Evaluation of serum Hcy levels forms an important feature to look further into molecular aspects.
The aim of this study was to evaluate the Hcy levels in NSCL ± P cases by comparing with control cases having no orofacial deformities.
This study was performed with a biochemical assay in a research laboratory.
A cross-sectional prevalence study was done to compare the concentrations of Hcy between 25 NSCL ± P patients and 15 healthy controls. Blood samples were collected from both the patients and controls and assessed for serum Hcy level using competent chemiluminescent immunoassay technique.
Student's -test was used for statistical analysis.
The average Hcy concentration was 9.5 μmol/L in control group. There was an increase in Hcy concentration among the NSCL ± P cases with an average value of 18.4 μmol/L. The results were found to be statistically significant using Student's -test.
The results of this study indicate that Hcy concentration has a significant elevation in NSCL ± P patients when compared with that of control cases.
非综合征性唇裂伴或不伴腭裂（NSCL±P）是一种遗传易感性疾病，涉及唇和腭的形状及结构缺陷。同型半胱氨酸（Hcy）水平升高见于多种医学并发症，如导致神经管缺陷的发育异常、先天性血管疾病、神经退行性疾病和精神疾病。评估血清Hcy水平是进一步研究分子层面的一个重要特征。
本研究的目的是通过与无口腔面部畸形的对照病例进行比较，评估NSCL±P病例中的Hcy水平。
本研究在一个研究实验室中采用生化检测方法进行。
进行了一项横断面患病率研究，以比较25例NSCL±P患者和15例健康对照者之间Hcy的浓度。从患者和对照者中采集血样，并使用可靠的化学发光免疫测定技术评估血清Hcy水平。
采用学生t检验进行统计分析。
对照组的平均Hcy浓度为9.5μmol/L。NSCL±P病例中的Hcy浓度有所升高，平均值为18.4μmol/L。使用学生t检验发现结果具有统计学意义。
本研究结果表明，与对照病例相比，NSCL±P患者的Hcy浓度显著升高。
