Stubbington Rachel, Dole-Olivier Marie-José, Galassi Diana M P, Hogan John-Paul, Wood Paul J
School of Science and Technology, Nottingham Trent University, Nottingham, Nottinghamshire, United Kingdom.
Université de Lyon, Université Claude Bernard Lyon 1, Villeurbanne Cedex, France.
PLoS One. 2016 Oct 10;11(10):e0164372. doi: 10.1371/journal.pone.0164372. eCollection 2016.
The hyporheic zone of river ecosystems provides a habitat for a diverse macroinvertebrate community that makes a vital contribution to ecosystem functioning and biodiversity. However, effective methods for sampling this community have proved difficult to establish, due to the inaccessibility of subsurface sediments. The aim of this study was to compare the two most common semi-quantitative macroinvertebrate pump-sampling techniques: Bou-Rouch and vacuum-pump sampling. We used both techniques to collect replicate samples in three contrasting temperate-zone streams, in each of two biogeographical regions (Atlantic region, central England, UK; Continental region, southeast France). Results were typically consistent across streams in both regions: Bou-Rouch samples provided significantly higher estimates of taxa richness, macroinvertebrate abundance, and the abundance of all UK and eight of 10 French common taxa. Seven and nine taxa which were rare in Bou-Rouch samples were absent from vacuum-pump samples in the UK and France, respectively; no taxon was repeatedly sampled exclusively by the vacuum pump. Rarefaction curves (rescaled to the number of incidences) and non-parametric richness estimators indicated no significant difference in richness between techniques, highlighting the capture of more individuals as crucial to Bou-Rouch sampling performance. Compared to assemblages in replicate vacuum-pump samples, multivariate analyses indicated greater distinction among Bou-Rouch assemblages from different streams, as well as significantly greater consistency in assemblage composition among replicate Bou-Rouch samples collected in one stream. We recommend Bou-Rouch sampling for most study types, including rapid biomonitoring surveys and studies requiring acquisition of comprehensive taxon lists that include rare taxa. Despite collecting fewer macroinvertebrates, vacuum-pump sampling remains an important option for inexpensive and rapid sample collection.
河流生态系统的潜流带为多样的大型无脊椎动物群落提供了栖息地，这对生态系统功能和生物多样性做出了至关重要的贡献。然而，由于地下沉积物难以获取，已证明难以建立有效的该群落采样方法。本研究的目的是比较两种最常见的半定量大型无脊椎动物泵吸采样技术：布-鲁什采样法和真空泵采样法。我们使用这两种技术在两个生物地理区域（大西洋地区，英国英格兰中部；大陆地区，法国东南部）的三条不同温带溪流中采集重复样本。两个区域的溪流结果通常一致：布-鲁什采样法对分类单元丰富度、大型无脊椎动物丰度以及所有英国常见分类单元和10个法国常见分类单元中的8个的丰度估计值显著更高。在英国和法国，分别有7个和9个在布-鲁什采样法样本中罕见的分类单元在真空泵采样法样本中未出现；没有分类单元仅被真空泵反复采样。稀疏曲线（重新缩放到出现次数）和非参数丰富度估计器表明，两种技术在丰富度上没有显著差异，这突出了捕获更多个体对布-鲁什采样性能的关键作用。与重复真空泵采样法样本中的群落相比，多变量分析表明，来自不同溪流的布-鲁什采样法群落之间差异更大，并且在一条溪流中采集的重复布-鲁什采样法样本之间群落组成的一致性也显著更高。我们建议对于大多数研究类型采用布-鲁什采样法，包括快速生物监测调查以及需要获取包含稀有分类单元的全面分类单元列表的研究。尽管采集到的大型无脊椎动物较少，但真空泵采样法仍然是廉价且快速采集样本的重要选择。
