Université Lyon, Université Lyon1, CNRS, INSA, CPE-Lyon, ICBMS, UMR 5246 , 43, Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
Anal Chem. 2016 Nov 1;88(21):10767-10772. doi: 10.1021/acs.analchem.6b03426. Epub 2016 Oct 21.
Three-dimensional (3D) printing technologies will impact the biosensor community in the near future, at both the sensor prototyping level and the sensing layer organization level. The present study aimed at demonstrating the capacity of one 3D printing technique, digital light processing (DLP), to produce hydrogel sensing layers with 3D shapes that are unattainable using conventional molding procedures. The first model of the sensing layer was composed of a sequential enzymatic reaction (glucose oxidase and peroxidase), which generated a chemiluminescent signal in the presence of glucose and luminol. Highly complex objects with assembly properties (fanciful ball, puzzle pieces, 3D pixels, propellers, fluidic and multicompartments) with mono-, di-, and tricomponents configurations were achieved, and the activity of the entrapped enzymes was demonstrated. The second model was a sandwich immunoassay protocol for the detection of brain natriuretic peptide. Here, highly complex propeller shape sensing layers were produced, and the recognition capability of the antibodies was elucidated. The present study opens then the path to a totally new field of development of multiplex sensing layers, printed separately and assembled on demand to create complex sensing systems.
三维（3D）打印技术将在不久的将来对生物传感器领域产生影响，无论是在传感器原型制作层面还是在传感层组织层面。本研究旨在展示一种 3D 打印技术，即数字光处理（DLP），生产具有 3D 形状的水凝胶传感层的能力，而这些形状是传统成型工艺无法实现的。传感层的第一个模型由顺序酶反应（葡萄糖氧化酶和过氧化物酶）组成，在存在葡萄糖和鲁米诺的情况下会产生化学发光信号。实现了具有组装特性（奇特的球、拼图、3D 像素、螺旋桨、流体和多腔室）的高度复杂的物体，并且证明了包埋酶的活性。第二个模型是用于检测脑钠肽的夹心免疫分析协议。在这里，生产出了具有高度复杂螺旋桨形状的传感层，并阐明了抗体的识别能力。本研究为多通道传感层的全新发展领域开辟了道路，这些传感层可以单独打印并按需组装，以创建复杂的传感系统。
