Li Fang, Ma Wenjing, Liu Jiachang, Wu Xiang, Wang Yan, He Jianbo
Anhui Province Key Laboratory of Advanced Catalytic Materials and Reaction Engineering, School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, Anhui, 230009, China.
Anal Bioanal Chem. 2018 Jan;410(2):543-552. doi: 10.1007/s00216-017-0752-5. Epub 2017 Nov 22.
Luminol, horseradish peroxidase (HRP), and glucose oxidase (GOx) ternary functionalized graphene oxide (HRP/GOx-luminol-GO) with excellent chemiluminescence (CL) activity and specific enzymatic property was prepared via a simple and general strategy for the first time. In this approach, luminol functionalized GO (luminol-GO) was prepared by gently stirring GO with luminol. Then HRP and GOx were further co-immobilized onto the surface of luminol-GO by storing HRP and GOx with luminol-GO at 4 °C overnight, to form HRP/GOx-luminol-GO bionanocomposites. The synthesized HRP/GOx-luminol-GO could react with HO generated from GOx catalyzed glucose oxidization reaction, to produce strong CL emission in the presence of co-immobilized HRP. Thus, we developed an ultrasensitive, homogeneous, reagentless, selective, and simple CL sensing system for glucose detection. The resulting biosensors exhibited ultra-wide linear range from 5.0 nM to 5.0 mM, and an ultra-low detection limit of 1.2 nM, which was more than 3 orders of magnitude lower than previously reported methods. Furthermore, the sensing system was successfully applied for the detection of glucose in human blood samples.
首次通过一种简单通用的策略制备了具有优异化学发光（CL）活性和特定酶促性质的鲁米诺、辣根过氧化物酶（HRP）和葡萄糖氧化酶（GOx）三元功能化氧化石墨烯（HRP/GOx-鲁米诺-GO）。在该方法中，通过将氧化石墨烯与鲁米诺轻轻搅拌制备鲁米诺功能化的氧化石墨烯（鲁米诺-GO）。然后，通过将HRP和GOx与鲁米诺-GO在4℃下储存过夜，将HRP和GOx进一步共固定在鲁米诺-GO的表面，以形成HRP/GOx-鲁米诺-GO生物纳米复合材料。合成的HRP/GOx-鲁米诺-GO可以与GOx催化葡萄糖氧化反应产生的HO反应，在共固定的HRP存在下产生强烈的CL发射。因此，我们开发了一种用于葡萄糖检测的超灵敏、均相、无试剂、选择性且简单的CL传感系统。所得生物传感器表现出5.0 nM至5.0 mM的超宽线性范围和1.2 nM的超低检测限，比先前报道的方法低3个多数量级。此外，该传感系统成功应用于人体血液样本中葡萄糖的检测。
