Rogers J M, Burkhead L M, Barbee B D
Perinatal Toxicology Branch, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711.
Teratology. 1989 Jun;39(6):515-23. doi: 10.1002/tera.1420390602.
The morphology of otoliths in CD-1 mouse and Syrian hamster fetuses exposed to the fungicide dinocap were evaluated at the end of gestation. Pregnant mice were dosed by gavage with 0, 10, 15, 30, or 60 mg/kg/day dinocap in corn oil on days 7-16 of gestation. Pregnant hamsters were dosed by the same route with 0, 50, 100, or 200 mg/kg/day on days 7-14 of gestation. At the end of gestation (day 18 in mice, day 15 in hamsters) dams were killed and all fetuses were removed and fixed overnight in 70% ethanol. Fetal heads were then removed, left in 70% ethanol for at least 3 days, and then dehydrated in a graded ethanol series and cleared with methyl salicylate. Otoliths were examined by darkfield microscopy, and each otolith was scored for morphological completeness on a scale of 0 to 3. Otolith development was complete by day 18 of gestation in control mouse fetuses. Otolith development was complete in many, but not all, of the hamster fetuses by day 15 of gestation. In the mouse, dinocap exposure inhibited fetal otolith formation in a dose-related manner, with a significant effect on total otolith score occurring at 10 mg/kg/day and above. Dinocap affected otolith formation in the hamster only at 100 mg/kg/day (200 mg/kg/day was embryolethal), concomitant with severe maternotoxicity and fetotoxicity.(ABSTRACT TRUNCATED AT 250 WORDS)
在妊娠期结束时，对接触杀真菌剂地乐酚的CD - 1小鼠和叙利亚仓鼠胎儿的耳石形态进行了评估。妊娠第7至16天，给怀孕小鼠经口灌胃给予0、10、15、30或60毫克/千克/天的地乐酚（溶于玉米油中）。妊娠第7至14天，给怀孕仓鼠经相同途径给予0、50、100或200毫克/千克/天。在妊娠期结束时（小鼠为第18天，仓鼠为第15天），处死母鼠，取出所有胎儿并在70%乙醇中固定过夜。然后取出胎儿头部，在70%乙醇中放置至少3天，接着在梯度乙醇系列中脱水，并用冬青油透明处理。通过暗视野显微镜检查耳石，每个耳石根据形态完整性在0至3的量表上评分。对照小鼠胎儿在妊娠第18天时耳石发育完成。许多（但并非所有）仓鼠胎儿在妊娠第15天时耳石发育完成。在小鼠中，地乐酚暴露以剂量相关的方式抑制胎儿耳石形成，在10毫克/千克/天及以上时对总耳石评分有显著影响。地乐酚仅在100毫克/千克/天（200毫克/千克/天具有胚胎致死性）时影响仓鼠的耳石形成，同时伴有严重的母体毒性和胚胎毒性。（摘要截短至250字）
