Lehane M J
School of Animal Biology, University of Wales, Bangor, Gwynedd, UK.
Tissue Cell. 1989;21(1):101-11. doi: 10.1016/0040-8166(89)90025-6.
The opaque zone cells of the midgut of the stablefly, Stomoxys calcitrans display a cyclical series of ultrastructural events in response to feeding, which it has been suggested are related to the synthesis and secretion of digestive enzymes. These cells have been studied in vivo using a combination of biochemical, morphometric and electron microscopical autoradiographic techniques. The cyclical nature, timing and relationship of the ultrastructural events to enzyme secretion has been confirmed. The autoradiographic data presented is in good agreement with the classical synthetic pathway for exported proteins. The kinetics of the cellular process have been described: transfer of newly synthesized product from the rough endoplasmic reticulum to Golgi apparatus begins ca. 13-14 min after labelling of the fly and from the Golgi apparatus to secretory granules after ca. 24-26 min. Secretion of this newly synthesized material begins before 60 min and possibly as early as 30 min after labelling of the fly. The data are discussed in relation to the comparable studies in other tissues.
厩螫蝇（Stomoxys calcitrans）中肠的不透明区细胞在进食后会呈现出一系列周期性的超微结构变化，有人认为这些变化与消化酶的合成和分泌有关。已经使用生化、形态计量学和电子显微镜放射自显影技术相结合的方法对这些细胞进行了体内研究。超微结构变化的周期性、时间以及与酶分泌的关系已得到证实。所呈现的放射自显影数据与经典的分泌蛋白合成途径高度一致。已经描述了细胞过程的动力学：新合成产物从糙面内质网转移到高尔基体大约在果蝇标记后13 - 14分钟开始，从高尔基体转移到分泌颗粒大约在24 - 26分钟后。这种新合成物质的分泌在果蝇标记后60分钟之前开始，可能早在30分钟就开始了。将结合其他组织的类似研究对这些数据进行讨论。
