中耳积液患儿中白细胞介素-17和22信使核糖核酸表达增加。
Increased IL-17 and 22 mRNA expression in pediatric patients with otitis media with effusion.
作者信息
Kwon Oh Eun, Park Sang Hyun, Kim Sung Su, Shim Haeng Seon, Kim Min Gyeong, Kim Young Il, Kim Sang Hoon, Yeo Seung Geun
机构信息
Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Kyung Hee University, Seoul, South Korea.
Department of Biochemistry and Molecular Biology, Medical Science and Engineering Research Center for Bioreaction to Reactive Oxygen Species, BK-21, School of Medicine, Kyung Hee University, Seoul, 130-701, South Korea.
出版信息
Int J Pediatr Otorhinolaryngol. 2016 Nov;90:188-192. doi: 10.1016/j.ijporl.2016.09.025. Epub 2016 Sep 21.
OBJECTIVES
Middle ear effusion has been reported to be associated with immune responses in patients with otitis media with effusion (OME). Although various cytokines are involved in immunologic responses in patients with OME, no study to date has assessed the involvement of the pro-inflammatory cytokines interleukin (IL)-17 and IL-22. This study analyzed the levels of expression of IL-17 and IL-22 in the middle ear effusion of patients with OME.
METHODS
Patients aged <11 years who were diagnosed with chronic OME and underwent ventilation tube insertion from May 2013 to August 2015 were enrolled. Effusion fluid samples were obtained during surgery and levels of IL-17 and IL-22 mRNAs assessed by real-time PCR. IL-17 and IL-22 mRNA levels were compared in patients with effusion fluid positive and negative for bacteria; in patients with and without accompanying diseases, recurrent disease, and re-operation; and relative to fluid characteristics.
RESULTS
The study cohort included 70 pediatric patients, 46 boys and 24 girls, of mean age 4.31 ± 2.11 years. The levels of IL-17 and IL-22 mRNA were higher in patients with than without sinusitis, but only IL-22 mRNA levels differed significantly (p < 0.05). The level of IL-17 mRNA was significantly higher in patients who did than did not undergo T&A (p < 0.05). The level of IL-22 expression was significantly higher in mucoid and purulent middle ear fluid samples than in serous fluid samples (p < 0.05).
CONCLUSION
IL-17 and IL-22 mRNAs are involved in the pathophysiology of OME and are significantly higher in subjects with than without accompanying diseases.
目的
据报道，中耳积液与分泌性中耳炎（OME）患者的免疫反应有关。尽管多种细胞因子参与了OME患者的免疫反应，但迄今为止，尚无研究评估促炎细胞因子白细胞介素（IL）-17和IL-22的参与情况。本研究分析了OME患者中耳积液中IL-17和IL-22的表达水平。
方法
纳入2013年5月至2015年8月期间诊断为慢性OME并接受通气管插入术的11岁以下患者。在手术期间获取积液样本，并通过实时PCR评估IL-17和IL-22 mRNA的水平。比较细菌检测积液阳性和阴性患者、伴有或不伴有伴随疾病、复发性疾病和再次手术患者的IL-17和IL-22 mRNA水平，并与积液特征进行比较。
结果
研究队列包括70名儿科患者，46名男孩和24名女孩，平均年龄4.31±2.11岁。有鼻窦炎的患者IL-17和IL-22 mRNA水平高于无鼻窦炎的患者，但只有IL-22 mRNA水平差异有统计学意义（p<0.05）。接受扁桃体和腺样体切除术（T&A）的患者IL-17 mRNA水平显著高于未接受T&A的患者（p<0.05）。黏液样和脓性中耳积液样本中IL-22表达水平显著高于浆液性积液样本（p<0.05）。
结论
IL-17和IL-22 mRNA参与了OME的病理生理过程，在伴有伴随疾病的患者中显著高于无伴随疾病的患者。
Zotero 插件