通过免疫磁珠分离和聚合酶链式反应相结合的方法检测鸡体内沙门氏菌的研究进展。
The development of methods for the detection of Salmonella in chickens by a combination of immunomagnetic separation and PCRs.
作者信息
Dai Fengying, Zhang Miao, Xu Dixin, Yang Yin, Wang Jiaxiao, Li Mingzhen, Du Meihong
机构信息
Key Laboratory of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Engineering Research Center of Food Safety Analysis, Beijing Center for Physical and Chemical Analysis, Beijing, People's Republic of China.
Beijing Scientific Instruments and Materials Cooperation, Beijing, People's Republic of China.
出版信息
Biotechnol Appl Biochem. 2017 Nov;64(6):888-894. doi: 10.1002/bab.1539. Epub 2017 Feb 7.
Micro- and nanoimmunomagnetic beads (MIMBs and NIMBs) used for immunomagnetic separation (IMS) with PCR were studied for the rapid detection of Salmonella. The capture efficiency of the two different IMBs was evaluated by a conventional plate counting method, and the binding pattern was studied using scanning electron microscopy. The specificity of the IMBs was tested with Salmonella, Shigella flexneri, enterohemorrhagic Escherichia coli O157:H7, and Listeria monocytogenes. By comparing the pre-enrichment IMS and the IMS enrichment steps with a 5.5-H enrichment time, this study developed a rapid and sensitive method for the detection of Salmonella in chicken. The method was implemented by IMS enrichment and PCR with MIMBs and NIMBs, with a total analysis time of 8 H. We showed that the method was sensitive based on NIMBs with a detection limit of 10° CFU for Salmonella in 25 g of chicken.
研究了用于聚合酶链反应（PCR）免疫磁珠分离（IMS）的微米和纳米免疫磁珠（MIMBs和NIMBs），以快速检测沙门氏菌。通过传统平板计数法评估两种不同免疫磁珠的捕获效率，并使用扫描电子显微镜研究结合模式。用沙门氏菌、福氏志贺氏菌、肠出血性大肠杆菌O157:H7和单核细胞增生李斯特菌测试免疫磁珠的特异性。通过比较预富集IMS和5.5小时富集时间的IMS富集步骤，本研究开发了一种快速灵敏的检测鸡肉中沙门氏菌的方法。该方法通过使用MIMBs和NIMBs进行IMS富集和PCR来实施，总分析时间为8小时。我们表明，基于NIMBs的方法具有敏感性，25克鸡肉中沙门氏菌的检测限为10⁰CFU。
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