Wang Yue J, Golson Maria L, Schug Jonathan, Traum Daniel, Liu Chengyang, Vivek Kumar, Dorrell Craig, Naji Ali, Powers Alvin C, Chang Kyong-Mi, Grompe Markus, Kaestner Klaus H
Department of Genetics and Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Medical Research, Corporal Michael J. Crescenz Veterans Affairs Medical Center and Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Cell Metab. 2016 Oct 11;24(4):616-626. doi: 10.1016/j.cmet.2016.09.007.
The human endocrine pancreas consists of multiple cell types and plays a critical role in glucose homeostasis. Here, we apply mass cytometry technology to measure all major islet hormones, proliferative markers, and readouts of signaling pathways involved in proliferation at single-cell resolution. Using this innovative technology, we simultaneously examined baseline proliferation levels of all endocrine cell types from birth through adulthood, as well as in response to the mitogen harmine. High-dimensional analysis of our marker protein expression revealed three major clusters of beta cells within individuals. Proliferating beta cells are confined to two of the clusters.
人类内分泌胰腺由多种细胞类型组成，在葡萄糖稳态中起关键作用。在这里，我们应用质谱流式细胞术技术，以单细胞分辨率测量所有主要胰岛激素、增殖标志物以及参与增殖的信号通路的读数。利用这项创新技术，我们同时检测了从出生到成年的所有内分泌细胞类型的基线增殖水平，以及对促细胞分裂剂 harmine 的反应。对我们的标记蛋白表达进行的高维分析揭示了个体内β细胞的三个主要簇。增殖的β细胞局限于其中两个簇。
