Bercovich Z, Lagendijk W, Bokhout B A
Immunology Department, Central Veterinary Institute, Lelystad, The Netherlands.
Vet Immunol Immunopathol. 1989 Jun;21(2):213-8. doi: 10.1016/0165-2427(89)90069-x.
The delayed-type hypersensitivity (DTH) test was used to diagnose brucellosis in two cows experimentally induced with brucellosis, and 176 dairy cows from a farm suspected of brucellosis. DTH test results were compared with results of the milk ring test, the serum agglutination test, the complement fixation test and the Coombs test. Cows positive in the DTH test and in one of the other tests were examined bacteriologically. In experimentally infected animals the DTH test was positive 10 days after infection, 1-4 weeks before serologic tests indicated brucellosis. Although the DTH test was positive during the whole experiment, on the one occasion when serologic titres were high, it was negative. Of the 176 dairy cows, 45 were positive in one or more serologic tests. In twelve cows (29%) the diagnosis was inconclusive because they were positive in only one of the serologic tests. In these cases the DTH test confirmed the infection. Three cows with high serologic response tested negative in the DTH test. B. abortus was isolated from 13 of 15 cows examined. We conclude that when serologic results are ambiguous, the DTH test is a useful additional technique for diagnosing brucellosis.
采用迟发型超敏反应（DTH）试验对两头实验性感染布鲁氏菌病的奶牛以及来自一个疑似布鲁氏菌病农场的176头奶牛进行布鲁氏菌病诊断。将DTH试验结果与乳环试验、血清凝集试验、补体结合试验和库姆斯试验结果进行比较。对DTH试验呈阳性且在其他试验之一中呈阳性的奶牛进行细菌学检查。在实验感染的动物中，感染后10天DTH试验呈阳性，比血清学试验显示布鲁氏菌病早1 - 4周。尽管在整个实验过程中DTH试验呈阳性，但在血清学滴度较高的一次检测中，它呈阴性。在176头奶牛中，45头在一项或多项血清学试验中呈阳性。12头奶牛（29%）的诊断结果不明确，因为它们仅在一项血清学试验中呈阳性。在这些情况下，DTH试验证实了感染。3头血清学反应高的奶牛DTH试验呈阴性。在接受检查的15头奶牛中，有13头分离出了流产布鲁氏菌。我们得出结论，当血清学结果不明确时，DTH试验是诊断布鲁氏菌病的一种有用的辅助技术。
