Bercovich Z, Dekker T, Eger A, Haagsma J
Department of Bacteriology, Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands.
Vet Res Commun. 1996;20(2):141-51. doi: 10.1007/BF00385635.
The potency of Brucella allergens prepared from a smooth Brucella abortus strain S-99, mucoid strain Leewarden, rough strain 45/20, and rough Brucella melitensis strain B-115 was assessed. The potency of these allergens was compared with that of a standard allergen prepared from smooth Brucella abortus S-99 that efficiently detected bovine brucellosis in other studies. Eight cattle experimentally inoculated with Brucella abortus 544 were tested with the allergens 4 and 10 weeks after infection, and again 8 months after infection. All the allergens effectively detected infection but there was a clear distinction in the mean skin reactions 48 and 72 h after injection of the allergens. The skin reactions provoked by the allergens prepared from smooth or mucoid strains of Brucella were most pronounced 48 h after injection. Skin reactions provoked by allergens prepared from rough strains of Brucella were strongest 72 h after injection. Allergens prepared from smooth or mucoid Brucella strains were more potent in detecting brucellosis than those prepared from rough strains of Brucella.
对从光滑型流产布鲁氏菌菌株S-99、黏液型菌株利尔瓦登、粗糙型菌株45/20以及粗糙型马尔他布鲁氏菌菌株B-115制备的布鲁氏菌变应原的效价进行了评估。将这些变应原的效价与在其他研究中能有效检测牛布鲁氏菌病的、由光滑型流产布鲁氏菌S-99制备的标准变应原的效价进行了比较。对8头经流产布鲁氏菌544实验性接种的牛，在感染后4周和10周以及感染后8个月用这些变应原进行了检测。所有变应原均能有效检测出感染，但在注射变应原后48小时和72小时的平均皮肤反应上存在明显差异。由光滑型或黏液型布鲁氏菌菌株制备的变应原引起的皮肤反应在注射后48小时最为明显。由粗糙型布鲁氏菌菌株制备的变应原引起的皮肤反应在注射后72小时最强。由光滑型或黏液型布鲁氏菌菌株制备的变应原在检测布鲁氏菌病方面比由粗糙型布鲁氏菌菌株制备的变应原更有效。
