猪脂肪来源祖细胞的鉴定与特性分析
Identification and characterization of pig adipose-derived progenitor cells.
作者信息
Zhang Shuang, Bai Chunyu, Zheng Dong, Gao Yuhua, Fan Yanan, Li Lu, Guan Weijun, Ma Yuehui
机构信息
College of Wildlife Resources, Northeast Forestry University, Harbin, P. R. China (Zhang, Zheng, Gao, Fan); Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (Bai, Gao, Fan, Li, Guan, Ma).
出版信息
Can J Vet Res. 2016 Oct;80(4):309-317.
Adipose-derived stem cells (ADSCs) are multipotent, and can be differentiated into many cell types In this study, tissues from pigs were chosen to identify and characterize ADSCs. Primary ADSCs were sub-cultured to passage 28. The surface markers of ADSCs: CD29, CD71, CD73, CD90, and CD166 were detected by reverse-transcription polymerase chain reaction assays and the markers CD29, CD44, CD105, and vimentin were detected by immunofluorescence. Growth curves and the capacity of clone-forming were performed to test the proliferation of ADSCs. Karyotype analysis showed that ADSCs cultured were genetically stable. To assess the differentiation capacity of the ADSCs, cells were induced to differentiate into osteoblasts, adipocytes, epithelial cells, neural cells, and hepatocyte-like cells. The results suggest that ADSCs from pigs showed similar biological characteristics with those separated from other species, and their multi-lineage differentiation shows potential as an application for cellular therapy in an animal model.
脂肪来源干细胞（ADSCs）具有多能性，可分化为多种细胞类型。在本研究中，选用猪的组织来鉴定和表征ADSCs。原代ADSCs传代培养至第28代。通过逆转录聚合酶链反应检测ADSCs的表面标志物：CD29、CD71、CD73、CD90和CD166，并通过免疫荧光检测标志物CD29、CD44、CD105和波形蛋白。绘制生长曲线并进行克隆形成能力检测以测试ADSCs的增殖情况。核型分析表明培养的ADSCs基因稳定。为评估ADSCs的分化能力，将细胞诱导分化为成骨细胞、脂肪细胞、上皮细胞、神经细胞和肝细胞样细胞。结果表明，猪源ADSCs与从其他物种分离的ADSCs具有相似的生物学特性，其多谱系分化在动物模型的细胞治疗应用中显示出潜力。
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