Lu Tengfei, Pei Wenhua, Wang Kunfu, Zhang Shuang, Chen Fenghao, Wu Yangnan, Guan Weijun
Department of Animal Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, P.R. China.
College of Wildlife Resources, Northeast Forestry University, Harbin, Heilongjiang 150040, P.R. China.
Exp Ther Med. 2018 Sep;16(3):2399-2407. doi: 10.3892/etm.2018.6445. Epub 2018 Jul 16.
In the past 10 years, adipose-derived stem cells (ADSCs) have been applied due to their pluripotency. Experimental tissues have been frequently obtained from mammals, including rabbits, mice and humans, but rarely from broilers, . In the present study, ADSCs were obtained from 20-day-old broiler embryos. Primary ADSCs were sub-cultured to passage 37 . The surface markers of ADSCs, namely CD29, CD31, CD44, CD71 and CD73, were detected by reverse transcription polymerase chain reaction and immunofluorescence assays. The result indicated that CD29, CD44, CD71 and CD73 were expressed on the surface of cells at various passages, but not CD31. The growth curve of cells at the different passages had a typical sigmoidal shape. Furthermore, ADSCs were successfully induced to differentiate into osteoblasts, adipocytes and hepatocyte-like cells. The results denote that the ADSCs isolated from broilers have similar biological properties to those of ADSCs obtained from other animals. The present study provided a theoretical and experimental foundation for the use of poultry as a source of stem cells, and laid a foundation for adipose tissue engineering and strategies in regenerative medicine.
在过去10年中，脂肪来源干细胞（ADSCs）因其多能性而得到应用。实验组织通常取自包括兔子、小鼠和人类在内的哺乳动物，但很少取自肉鸡。在本研究中，ADSCs取自20日龄的肉鸡胚胎。原代ADSCs传代培养至第37代。通过逆转录聚合酶链反应和免疫荧光分析检测ADSCs的表面标志物，即CD29、CD31、CD44、CD71和CD73。结果表明，CD29、CD44、CD71和CD73在不同传代的细胞表面均有表达，但CD31未表达。不同传代细胞的生长曲线呈典型的S形。此外，ADSCs成功诱导分化为成骨细胞、脂肪细胞和类肝细胞。结果表明，从肉鸡分离的ADSCs与从其他动物获得的ADSCs具有相似的生物学特性。本研究为将家禽作为干细胞来源提供了理论和实验基础，并为脂肪组织工程和再生医学策略奠定了基础。
