Palange Padmavali, Narang Rahul, Kandi Venkataramana
Department of Microbiology, Prathima Institute of Medical Sciences.
Department of Microbiology, Mahatma Gandhi institute of Medical Sciences, Sevagram, Maharashtra.
Cureus. 2016 Aug 30;8(8):e757. doi: 10.7759/cureus.757.
Laboratory diagnosis of tuberculosis has undergone a rapid change during last few years and a number of techniques for culture as well as molecular diagnosis have been used with their respective advantages and disadvantages. Sporadic studies have also reported the isolation of on standard blood agar (BA), which at one time was not considered as a suitable medium for mycobacterial culture. The present study was conducted to evaluate the routine use of 5% sheep BA in a mycobacteriology laboratory by comparing isolation rates and time for isolation of mycobacteria from pulmonary and extrapulmonary samples with those on Lowenstein-Jensen (LJ) medium.
BA with antibiotics was prepared and dispensed as slants in McCartney bottles. LJ was prepared and dispensed following the Revised National Tuberculosis Control Programme (RNTCP) guidelines. A total of 500 suspected tuberculosis samples were inoculated on both media in duplicate, incubated at 37C, and observed daily until the appearance of growth.
Out of 500 inoculated samples, 99 showed growth on BA and 112 showed growth on LJ medium. Mean growth time on BA was less as compared to LJ medium. The contamination rate was found to be more on BA (7.2%) than on LJ (4.8%).
Mycobacterial growth time was less on BA as compared to LJ.
在过去几年中，结核病的实验室诊断发生了迅速变化，多种培养技术以及分子诊断技术都有应用，各有其优缺点。零星研究也报道了在标准血琼脂（BA）上分离到结核菌，而血琼脂曾一度被认为不是适合分枝杆菌培养的培养基。本研究旨在通过比较从肺部和肺外样本中分离分枝杆菌的分离率和时间，评估5%羊血琼脂在分枝杆菌学实验室中的常规应用情况，与罗琴（LJ）培养基进行对比。
制备含抗生素的血琼脂，分装在麦氏瓶中制成斜面。按照修订后的国家结核病控制规划（RNTCP）指南制备并分装LJ培养基。总共500份疑似结核病样本分别一式两份接种于两种培养基上，在37℃孵育，每天观察直至出现生长。
在500份接种样本中，99份在血琼脂上生长，112份在LJ培养基上生长。与LJ培养基相比，血琼脂上的平均生长时间更短。发现血琼脂上的污染率（7.2%）高于LJ培养基（4.8%）。
与LJ培养基相比，分枝杆菌在血琼脂上的生长时间更短。
