Shimo Tsuyoshi, Yoshioka Norie, Takigawa Masaharu, Sasaki Akira
Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, Okayama, 700-8525, Japan.
Methods Mol Biol. 2017;1489:505-512. doi: 10.1007/978-1-4939-6430-7_42.
Bone metastasis is a common occurrence in human malignancies, including breast, prostate, and lung cancer, and is associated with a high morbidity rate because of intractable bone pain, pathological fractures, hypercalcemia, and nerve compression. Animal models of bone metastasis are important tools to investigate the pathogenesis and develop treatment strategies. However, there are few models of spontaneous bone metastasis despite the fact that animals often spontaneously develop cancer. Here, we describe methods for developing a mouse model of breast cancer bone metastasis achieved by injection of MDA-MB-231 breast cancer cells into the heart. This assay can be applied to studies on roles of CCN proteins in tumor metastasis and development of treatment strategies targeting CCN proteins.
骨转移在人类恶性肿瘤中很常见，包括乳腺癌、前列腺癌和肺癌，并且由于顽固性骨痛、病理性骨折、高钙血症和神经压迫而具有较高的发病率。骨转移动物模型是研究发病机制和制定治疗策略的重要工具。然而，尽管动物经常自发发生癌症，但自发骨转移模型却很少。在此，我们描述了通过将MDA-MB-231乳腺癌细胞注射到心脏来建立乳腺癌骨转移小鼠模型的方法。该试验可应用于研究CCN蛋白在肿瘤转移中的作用以及针对CCN蛋白的治疗策略的开发。
