Shimo Tsuyoshi, Okui Tatsuo, Horie Naohiro, Yokozeki Kenji, Takigawa Masaharu, Sasaki Akira
Division of Reconstructive Surgery for Oral and Maxillofacial Region, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan.
Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Methods Mol Biol. 2023;2582:343-353. doi: 10.1007/978-1-0716-2744-0_24.
Bone metastasis and bone destruction are common occurrences in human malignancies, including breast, prostate, and lung cancer, and are associated with a high morbidity rate because of intractable bone pain, pathological fractures, hypercalcemia, and nerve compression. Animal models of bone metastasis and bone destruction are important tools to investigate the pathogenesis and develop treatment strategies. However, there are few models of spontaneous bone metastasis despite the fact that animals often spontaneously develop cancer. Here, we describe methods for developing a mouse model of breast cancer bone metastasis achieved by injection of MDA-MB-231 breast cancer cells into the left cardiac ventricle. In addition, we introduce mouse model of the bone destruction by injection of SAS oral squamous cell carcinoma cells into the bone marrow space of the right tibial metaphysis. These assays can be applied to studies on roles of cellular communication network factor/connective tissue growth factor (CTGF/CCN2) protein in tumor metastasis and development of treatment strategies targeting CCN proteins.
骨转移和骨破坏在人类恶性肿瘤中很常见，包括乳腺癌、前列腺癌和肺癌，并且由于顽固性骨痛、病理性骨折、高钙血症和神经压迫而具有较高的发病率。骨转移和骨破坏的动物模型是研究发病机制和制定治疗策略的重要工具。然而，尽管动物经常自发发生癌症，但很少有自发骨转移的模型。在此，我们描述了通过将MDA-MB-231乳腺癌细胞注射到左心室来建立乳腺癌骨转移小鼠模型的方法。此外，我们介绍了通过将SAS口腔鳞状细胞癌细胞注射到右胫骨近端骨髓腔来建立骨破坏小鼠模型的方法。这些试验可应用于研究细胞通讯网络因子/结缔组织生长因子（CTGF/CCN2）蛋白在肿瘤转移中的作用以及针对CCN蛋白的治疗策略的开发。
