Luo Jia, Shen Yuan L, Lei Gang H, Zhu Ping K, Jiang Zhi Y, Bai Lin, Li Zhou M, Tang Qing G, Li Wei X, Zhang Hua S, Zhu Li
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G 2P5, Canada.
J Sci Food Agric. 2017 Jul;97(9):2706-2713. doi: 10.1002/jsfa.8094. Epub 2016 Nov 21.
The present study aimed to evaluate the correlations among muscle concentrations of three glycometabolic-related hormones (insulin, epinephrine and glucagon), muscle glycolysis and meat quality in representative muscles of either glycolytic or oxidative types. Moreover, the relative glycometabolic-related gene expression was measured. One Western crossbreed DLY (Duroc × (Landrace × Yorkshire)), one crossbreed with half-Chinese native-pig origin DL (Duroc × LiangShan) and one pure Chinese native pig TP (Tibetan pig) were used in the present study.
Among the three breeds, DLY had the greatest glucagon and epinephrine (P < 0.01). Compared with DLY, TP and DL had lower lactic acid concentrations, showing lower glycolytic potentials (GP), greater ultimate pH values (P < 0.01) and lower relative expression levels of glycometabolic-related genes (GYS1, PRKAG3 and PKM2). Compared with the glycolytic muscle (musculus longissimus dorsi), oxidative muscle PM (musculus psoas major) had lower glucagon and epinephrine contents, lower GP and better meat quality. The concentration of glycometabolic-related hormones in the muscle had significant correlations with muscle glycolysis, meat pH and lightness.
The results obtained in the present study imply that glucagon and epinephrine levels could be used to indicate early glycolytic metabolism during postmortem. These findings may be helpful in identifying pork with undesirable quality traits. 2016 Society of Chemical Industry.
本研究旨在评估三种糖代谢相关激素（胰岛素、肾上腺素和胰高血糖素）在肌肉中的浓度、肌肉糖酵解与糖酵解型或氧化型代表性肌肉肉质之间的相关性。此外，还测定了相对糖代谢相关基因的表达。本研究使用了一个西方杂交品种DLY（杜洛克×（长白猪×大白猪））、一个具有一半中国本地猪血统的杂交品种DL（杜洛克×凉山猪）和一头中国本地纯种猪TP（藏猪）。
在这三个品种中，DLY的胰高血糖素和肾上腺素含量最高（P < 0.01）。与DLY相比，TP和DL的乳酸浓度较低，表明糖酵解潜力（GP）较低，最终pH值较高（P < 0.01），糖代谢相关基因（GYS1、PRKAG3和PKM2）的相对表达水平较低。与糖酵解型肌肉（背最长肌）相比，氧化型肌肉PM（腰大肌）的胰高血糖素和肾上腺素含量较低，GP较低，肉质较好。肌肉中糖代谢相关激素的浓度与肌肉糖酵解、肉的pH值和亮度显著相关。
本研究结果表明，胰高血糖素和肾上腺素水平可用于指示宰后早期的糖酵解代谢。这些发现可能有助于识别具有不良品质特征的猪肉。2016年化学工业协会。
