Dige A, Rasmussen T K, Nejsum P, Hagemann-Madsen R, Williams A R, Agnholt J, Dahlerup J F, Hvas C L
Department of Hepatology and Gastroenterology, Aarhus University Hospital, Aarhus C, Denmark.
Department of Biomedicine, Aarhus University, Aarhus C, Denmark.
Parasite Immunol. 2017 Jan;39(1). doi: 10.1111/pim.12394. Epub 2016 Dec 14.
Helminthic therapy of immune-mediated diseases has gained attention in recent years, but we know little of how helminths modulate human immunity. In this study, we investigated how self-infection with Trichuris (T.) trichiura in an adult man without intestinal disease affected mucosal and systemic immunity. Colonic mucosal biopsies were obtained at baseline, during T. trichiura infection, and after its clearance following mebendazole treatment. Unexpectedly, the volunteer experienced a Campylobacter colitis following T. trichiura clearance, and this served as a positive infectious control. Trichuris trichiura colonization induced equally increased expressions of T-helper (h)1-, Th2-, Th17- and Treg-associated cytokines and transcription factors, measured by quantitative polymerase chain reaction. We observed several indicators of modulation of systemic immunity during the T. trichiura infection. Plasma eosinophils and anti-Trichuris antibodies rose markedly during the inoculation phase, and a shift towards a Th2-dominated T cell response at the expense of the Th1-response was observed in circulating T cells. Taken together, our findings corroborate that helminths modulate regional and systemic human immunity.
近年来，蠕虫疗法在免疫介导疾病的治疗中受到关注，但我们对蠕虫如何调节人体免疫了解甚少。在本研究中，我们调查了一名无肠道疾病的成年男性自我感染 Trichuris（T.）trichiura 如何影响黏膜和全身免疫。在基线、Trichuris trichiura 感染期间以及甲苯咪唑治疗清除感染后，获取结肠黏膜活检样本。出乎意料的是，志愿者在 Trichuris trichiura 清除后发生了弯曲杆菌性结肠炎，这作为一个阳性感染对照。通过定量聚合酶链反应测定，Trichuris trichiura 定植诱导 T 辅助（h）1、Th2、Th17 和 Treg 相关细胞因子及转录因子表达均增加。我们观察到 Trichuris trichiura 感染期间全身免疫调节的几个指标。接种阶段血浆嗜酸性粒细胞和抗 Trichuris 抗体显著升高，并且在循环 T 细胞中观察到以 Th1 反应为代价向 Th2 主导的 T 细胞反应转变。综上所述，我们的研究结果证实蠕虫可调节人体局部和全身免疫。
