Cao Xiaofang, Wa Qingbiao, Wang Qidi, Li Lin, Liu Xin, An Lisha, Cai Ruikun, Du Meng, Qiu Yue, Han Jian, Wang Chunlin, Wang Xingyu, Guo Changlong, Lu Yonghong, Ma Xu
Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China.
Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu 610017, China.
Int Immunopharmacol. 2016 Nov;40:487-491. doi: 10.1016/j.intimp.2016.10.004. Epub 2016 Oct 13.
Psoriasis is a T cell-mediated chronic inflammatory skin disease with inflammatory cell infiltrates in the dermis and epidermis. Previous studies suggested that there are some expanded T-cell receptor (TCR) clones in psoriatic skin. However, the effect of psoriasis on the immunological characteristics of TCR in circulating blood has not been reported. To address this, we performed high-throughput sequencing to reveal the immunological characteristics of TCR beta chain (TRB) in both psoriasis patients and healthy controls. Our results revealed that the TRB-CDR3 region of psoriasis patients had distinctive immunological characteristics compared with that of healthy controls, including V gene usage, nt of N addition. In addition, three types of TRB-CDR3 peptides were found highly relevant to psoriasis. Our findings show the comprehensive characteristics of psoriasis on the TRB-CDR3 repertoire of circulating blood at sequence-level resolution. These findings may contribute to a better understanding of the pathogenesis of psoriasis and open opportunities to explore potential therapeutic targets.
银屑病是一种由T细胞介导的慢性炎症性皮肤病，真皮和表皮中有炎症细胞浸润。先前的研究表明，银屑病皮肤中存在一些扩增的T细胞受体（TCR）克隆。然而，银屑病对循环血液中TCR免疫特征的影响尚未见报道。为了解决这一问题，我们进行了高通量测序，以揭示银屑病患者和健康对照中TCRβ链（TRB）的免疫特征。我们的结果显示，与健康对照相比，银屑病患者的TRB - CDR3区域具有独特的免疫特征，包括V基因使用、N添加的核苷酸。此外，发现三种类型的TRB - CDR3肽与银屑病高度相关。我们的研究结果在序列水平分辨率上显示了银屑病对循环血液中TRB - CDR3库的综合特征。这些发现可能有助于更好地理解银屑病的发病机制，并为探索潜在治疗靶点提供机会。
