Institute of Dermatology, Guangzhou Medical University, Guangzhou 510095, PR China; Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, PR China.
BGI Genomics, BGI-Shenzhen, Shenzhen 518083, PR China.
Mol Immunol. 2020 Apr;120:23-31. doi: 10.1016/j.molimm.2020.01.024. Epub 2020 Feb 8.
Acne is a common chronic inflammatory skin disease, and the inflammation immune response runs through all stages of acne lesions. In this study, we use a combination of multiplex-PCR and high-throughput sequencing technologies to analyze T cell receptor β chain CDR3 (complementarity-determining region 3) in peripheral blood isolated from severe acne patients. Once compared with healthy controls, we propose to identify acne-relevant CDR3 peptides. Our results reveal that the diversity of T cell receptor β chain (TRB) CDR3 sequences in the peripheral blood of the severe acne vulgaris (SA) group differed from that of the control group. In addition, we find 10 TRB CDR3 sequences, amino acid sequences and V-J combinations with significantly different expressions between the SA group and the non-acne (NA) group (P < 0.0001). These findings may contribute to a better understanding of the role of immunity in the pathogenesis of acne and may serve as biomarkers for evaluating risk or prognosis of severe acne disease in future.
痤疮是一种常见的慢性炎症性皮肤病，炎症免疫反应贯穿于痤疮皮损的各个阶段。在这项研究中，我们采用多重 PCR 和高通量测序技术，分析了来自严重痤疮患者外周血中的 T 细胞受体 β 链 CDR3（互补决定区 3）。一旦与健康对照组进行比较，我们就提出了识别痤疮相关 CDR3 肽的方法。我们的研究结果表明，严重寻常痤疮（SA）组外周血 T 细胞受体 β 链（TRB）CDR3 序列的多样性与对照组不同。此外，我们发现 10 个 TRB CDR3 序列，其氨基酸序列和 V-J 组合在 SA 组和非痤疮（NA）组之间的表达存在显著差异（P<0.0001）。这些发现可能有助于更好地理解免疫在痤疮发病机制中的作用，并可能成为评估严重痤疮疾病风险或预后的生物标志物。
