Catalá-Icardo Mónica, Gómez-Benito Carmen, Simó-Alfonso Ernesto Francisco, Herrero-Martínez José Manuel
Research Institute for Integrated Management of Coastal Areas, Universitat Politècnica de València, Paranimf 1, 46730, Grao de Gandía, Valencia, Spain.
Department of Analytical Chemistry, Universitat de València, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.
Anal Bioanal Chem. 2017 Jan;409(1):243-250. doi: 10.1007/s00216-016-9993-y. Epub 2016 Oct 15.
This paper describes a novel and sensitive method for extraction, preconcentration, and determination of two important widely used fungicides, azoxystrobin, and chlorothalonil. The developed methodology is based on solid-phase extraction (SPE) using a polymeric material functionalized with gold nanoparticles (AuNPs) as sorbent followed by high-performance liquid chromatography (HPLC) with diode array detector (DAD). Several experimental variables that affect the extraction efficiency such as the eluent volume, sample flow rate, and salt addition were optimized. Under the optimal conditions, the sorbent provided satisfactory enrichment efficiency for both fungicides, high selectivity and excellent reusability (>120 re-uses). The proposed method allowed the detection of 0.05 μg L of the fungicides and gave satisfactory recoveries (75-95 %) when it was applied to drinking and environmental water samples (river, well, tap, irrigation, spring, and sea waters).
本文描述了一种新颖且灵敏的方法，用于提取、预富集和测定两种重要且广泛使用的杀菌剂——嘧菌酯和百菌清。所开发的方法基于固相萃取（SPE），使用用金纳米颗粒（AuNPs）功能化的聚合材料作为吸附剂，随后采用配有二极管阵列检测器（DAD）的高效液相色谱（HPLC）。对影响萃取效率的几个实验变量，如洗脱液体积、样品流速和盐添加量进行了优化。在最佳条件下，该吸附剂对两种杀菌剂均提供了令人满意的富集效率、高选择性和出色的可重复使用性（>120次重复使用）。所提出的方法能够检测出0.05 μg/L的杀菌剂，应用于饮用水和环境水样（河水、井水、自来水、灌溉水、泉水和海水）时回收率令人满意（75 - 95%）。
