Liu Qingju, Han Ping, Wang Hui, Gong Wenwen, Feng Xiaoyuan
Beijing Research Center for Agriculture Standards and Testing Beijing 100097 China
Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture Beijing 100097 China.
RSC Adv. 2019 Mar 29;9(18):9893-9898. doi: 10.1039/c9ra00291j. eCollection 2019 Mar 28.
Currently known rapid determination of fungicides usually relies on antibody-based immunoassay. This paper reports a simple antibody-free colorimetric assay for chlorothalonil the inhibition of an enzyme-triggered reaction. The enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase was significantly inhibited by chlorothalonil, and the color change of NBT-PMS system induced from NADH formation was suppressed, which could be used indirectly to assay chlorothalonil. The limit of detection (LOD) was 0.05 μM with a linear range from 0.5 to 10 μM, and the detection of 1 μM chlorothalonil in solution was achieved with a naked-eye readout. In addition, the colorimetric measurement results of the cucumber samples showed a good recovery rate, although the sensitivity was less effective than the instrumental method. Nevertheless, the results demonstrates that the chlorometric method provides potential opportunities for reliable, cost-effective quantitative detection for chlorothalonil residues in vegetables.
目前已知的杀菌剂快速检测通常依赖基于抗体的免疫分析。本文报道了一种简单的无抗体比色法用于检测百菌清，该方法基于百菌清对酶触发反应的抑制作用。百菌清能显著抑制3-磷酸甘油醛脱氢酶的酶活性，抑制由NADH生成引起的NBT-PMS体系的颜色变化，从而可间接用于检测百菌清。检测限（LOD）为0.05 μM，线性范围为0.5至10 μM，可通过肉眼读数实现对溶液中1 μM百菌清的检测。此外，黄瓜样品的比色测量结果显示出良好的回收率，尽管灵敏度不如仪器方法有效。然而，结果表明比色法为蔬菜中百菌清残留的可靠、经济高效的定量检测提供了潜在机会。
