Wang Cuiping, Lin Xinying, Guo Dongmei, Ding Lili, Guo Haifeng, Xu Guifa, Cui Xi, Wang Xia
Department of Nutrition and Food Hygiene, School of Public Health, Shandong University, 44 Wenhuaxi Road, Jinan, 250012, China.
Department of Chemistry and Bacteria Detection, School of Public Health, Shandong University, Jinan, China.
Biol Trace Elem Res. 2017 May;177(1):16-24. doi: 10.1007/s12011-016-0865-2. Epub 2016 Oct 15.
The objective of this study was to investigate the zinc fractional absorption of young Chinese men and women from the Shandong rural region under the routine dietary pattern by stable isotope technique. Ten men and 10 women, aged 20 to 35 years, and with a representative diet during the experiment were recruited from the Shandong rural region. Stable Zn was used as a tracer to label ZnCl, and Yb was used to monitor the excretion of Zn in urine and feces. All volunteers were given rice containing 4.0 mg Zn and 1.0 mg Yb on the fourth day. Then the food and fecal samples of all subjects were collected for 12 consecutive days. The total zinc and the stable zinc isotope ratio of all samples were determined by atomic absorption spectrophotometer and thermal ionization mass spectrometer, respectively. The determination of the other nutrients was performed based on the Chinese National Standard Methods. Among volunteers, the daily intake of zinc was 15.50 mg, 103.33 % of recommended nutrient intake (RNI, set by the Chinese Nutrition Society) in men and 15.43 mg, 134.17 % in women. The fractional absorption of Zn was 23.42 ± 2.23 % in men, and 22.49 ± 2.19 % in women. The protein candidates got from the typical diets was 93.96 % of RNI in women. Calcium and ascorbic acid intakes were 76.23 % of RNI and 27.91 % of RNI in men, respectively and 51.17 % of RNI and 34.23 % of RNI in women, respectively. Our results showed that a typical meal for someone in the Shandong rural region presented an adequate intake of zinc and a moderate Zn bioavailability. The shortage of protein and the inappropriate protein pattern may play important roles in reducing zinc bioavailability.
本研究的目的是采用稳定同位素技术，调查山东农村地区青年男女在日常饮食模式下锌的分数吸收情况。从山东农村地区招募了10名男性和10名女性，年龄在20至35岁之间，且在实验期间饮食具有代表性。用稳定锌作为示踪剂标记氯化锌，用镱监测锌在尿液和粪便中的排泄情况。在第四天，给所有志愿者食用含4.0毫克锌和1.0毫克镱的大米。然后连续12天收集所有受试者的食物和粪便样本。分别用原子吸收分光光度计和热电离质谱仪测定所有样本的总锌和稳定锌同位素比率。其他营养素的测定按照中国国家标准方法进行。在志愿者中，男性锌的每日摄入量为15.50毫克，占推荐营养素摄入量（中国营养学会设定的RNI）的103.33%，女性为15.43毫克，占134.17%。男性锌的分数吸收为23.42±2.23%，女性为22.49±2.19%。从典型饮食中获取的蛋白质候选量在女性中占RNI的93.96%。男性钙和抗坏血酸的摄入量分别占RNI的76.23%和27.91%，女性分别占RNI的51.17%和34.23%。我们的结果表明，山东农村地区某人的一顿典型膳食锌摄入量充足，锌生物利用率适中。蛋白质缺乏和蛋白质模式不当可能在降低锌生物利用率方面起重要作用。
