Ribeiro M A, Cominetti C, Kakazu M H, Sarkis J E S, Dainty J, Fox T E, Cozzolino S M F
Nutrition Nucleus, Academic Center of Vitoria, Federal University of Pernambuco, Recife, Brazil.
J Hum Nutr Diet. 2014 Apr;27 Suppl 2:313-20. doi: 10.1111/jhn.12119. Epub 2013 May 2.
The high amounts of phytic acid present in diets from developing countries are considered as important inhibitors of zinc (Zn) absorption. The present study aimed to assess the fractional absorption of Zn from a meal containing common Brazilian foods using the stable isotope technique.
Twelve men, aged 19-42 years, were fed a healthy experimental diet comprising lettuce, tomato, French fries, steak with onions, rice, beans, papaya, orange, pineapple, and passion fruit juice. Each subject received one intravenous dose of enriched (70) Zn, and the lunch was extrinsically labelled with enriched (67) Zn. Urinary (67) Zn and (70) Zn enrichments were assessed by inductively coupled plasma mass spectrometry.
The labelled meal phytate : Zn molar ratio was very divergent with respect to chemically determined and calculated data. Subjects presented a normal Zn nutritional status before and after the study. The mean Zn absorption from the labelled meal was 30% (range 11-47%).
According to the World Health Organization parameters, the results denote a moderate/high Zn bioavailability in the evaluated meal, with a variability in the absorption percentage that is similar to other studies. The data show that a typical Brazilian meal, with an adequate energy amount and a balanced macronutrient distribution, presents a Zn bioavailability in accordance with the worldwide recommended standard.
发展中国家饮食中存在的大量植酸被认为是锌（Zn）吸收的重要抑制剂。本研究旨在使用稳定同位素技术评估从一顿包含常见巴西食物的餐食中锌的吸收分数。
12名年龄在19至42岁之间的男性食用了一种健康的实验性饮食，包括生菜、番茄、薯条、洋葱牛排、米饭、豆类、木瓜、橙子、菠萝和西番莲果汁。每位受试者接受一剂静脉注射的富集（70）Zn，午餐用富集（67）Zn进行外部标记。通过电感耦合等离子体质谱法评估尿液中（67）Zn和（70）Zn的富集情况。
标记餐食的植酸：锌摩尔比在化学测定数据和计算数据方面差异很大。研究前后受试者的锌营养状况正常。标记餐食中锌的平均吸收率为30%（范围为11 - 47%）。
根据世界卫生组织的参数，结果表明在评估的餐食中锌的生物利用率为中度/高度，吸收百分比的变异性与其他研究相似。数据表明，一顿能量充足且常量营养素分布均衡的典型巴西餐食，其锌生物利用率符合全球推荐标准。
