Abdul Manaf Shoriya Aruni, Hegde Gurumurthy, Mandal Uttam Kumar, Wui Tin Wong, Roy Partha
Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, 26300, Gambang, Kuantan. Malaysia.
BMS R and D Centre, BMSCE, Bull Temple Road, 560019, Bangalore. India.
Curr Drug Deliv. 2017;14(8):1071-1077. doi: 10.2174/1567201813666161017130612.
Nano-scale carbon systems are emerging alternatives in drug delivery and bioimaging applications of which they gradually replace the quantum dots characterized by toxic heavy metal content in the latter application.
The work intended to use carbon nanospheres synthesized from biowaste Sago bark for cancer cell imaging applications.
This study synthesised carbon nanospheres from biowaste Sago bark using a catalyst-free pyrolysis technique. The nanospheres were functionalized with fluorescent dye coumarin-6 for cell imaging. Fluorescent nanosytems were characterized by field emission scanning electron microscopy-energy dispersive X ray, photon correlation spectroscopy and fourier transform infrared spectroscopy techniques.
The average size of carbon nanospheres ranged between 30 and 40 nm with zeta potential of -26.8 ± 1.87 mV. The percentage viability of cancer cells on exposure to nanospheres varied from 91- 89 % for N2a cells and 90-85 % for A-375 cells respectively. Speedy uptake of the fluorescent nanospheres in both N2a and A-375 cells was observed within two hours of exposure.
Novel fluorescent carbon nanosystem design following waste-to-wealth approach exhibited promising potential in cancer cell imaging applications.
纳米级碳系统正在成为药物递送和生物成像应用中的替代方案，在后者的应用中它们逐渐取代了含有有毒重金属成分的量子点。
本研究旨在将由生物废弃物西米树皮合成的碳纳米球用于癌细胞成像应用。
本研究采用无催化剂热解技术从生物废弃物西米树皮中合成碳纳米球。用荧光染料香豆素-6对纳米球进行功能化处理以用于细胞成像。通过场发射扫描电子显微镜-能量色散X射线、光子相关光谱和傅里叶变换红外光谱技术对荧光纳米系统进行表征。
碳纳米球的平均尺寸在30至40纳米之间，zeta电位为-26.8±1.87毫伏。暴露于纳米球后，N2a细胞的癌细胞活力百分比分别为91%-89%，A-375细胞为90%-85%。在暴露两小时内观察到N2a和A-375细胞对荧光纳米球的快速摄取。
遵循变废为宝方法设计的新型荧光碳纳米系统在癌细胞成像应用中展现出了有前景的潜力。
