Hayashi Hisamitsu, Edin Fredrik, Li Hao, Liu Wei, Rask-Andersen Helge
Department of Surgical Sciences, Head and Neck Surgery, Section of Otolaryngology, Uppsala University Hospital, SE-751 85 Uppsala, Sweden; Department of Otolaryngology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
Department of Surgical Sciences, Head and Neck Surgery, Section of Otolaryngology, Uppsala University Hospital, SE-751 85 Uppsala, Sweden.
Brain Res. 2016 Dec 1;1652:195-203. doi: 10.1016/j.brainres.2016.09.043. Epub 2016 Oct 13.
Endogenous electric fields (EFs) are required for the physiological control of the central nervous system development. Application of the direct current EFs to neural stem cells has been studied for the possibility of stem cell transplantation as one of the therapies for brain injury. EFs generated within the nervous system are often associated with action potentials and synaptic activity, apparently resulting in a pulsed current in nature. The aim of this study is to investigate the effect of pulsed EF, which can reduce the cytotoxicity, on the migration of human neural progenitor cells (hNPCs). We applied the mono-directional pulsed EF with a strength of 250mV/mm to hNPCs for 6h. The migration distance of the hNPCs exposed to pulsed EF was significantly greater compared with the control not exposed to the EF. Pulsed EFs, however, had less of an effect on the migration of the differentiated hNPCs. There was no significant change in the survival of hNPCs after exposure to the pulsed EF. To investigate the role of Ca signaling in electrotactic migration of hNPCs, pharmacological inhibition of Ca channels in the EF-exposed cells revealed that the electrotactic migration of hNPCs exposed to Ca channel blockers was significantly lower compared to the control group. The findings suggest that the pulsed EF induced migration of hNPCs is partly influenced by intracellular Ca signaling.
内源性电场（EFs）是中枢神经系统发育生理控制所必需的。将直流电场应用于神经干细胞，作为脑损伤治疗方法之一的干细胞移植可能性已得到研究。神经系统内产生的电场通常与动作电位和突触活动相关，显然在本质上会产生脉冲电流。本研究的目的是研究可降低细胞毒性的脉冲电场对人神经祖细胞（hNPCs）迁移的影响。我们将强度为250mV/mm的单向脉冲电场施加于hNPCs 6小时。与未暴露于电场的对照组相比，暴露于脉冲电场的hNPCs的迁移距离显著更大。然而，脉冲电场对分化的hNPCs迁移的影响较小。暴露于脉冲电场后，hNPCs的存活率没有显著变化。为了研究钙信号在hNPCs趋电迁移中的作用，对暴露于电场的细胞中的钙通道进行药理学抑制，结果显示，与对照组相比，暴露于钙通道阻滞剂的hNPCs的趋电迁移显著降低。研究结果表明，脉冲电场诱导的hNPCs迁移部分受细胞内钙信号影响。
