Suárez-Esquivel Marcela, Ruiz-Villalobos Nazareth, Castillo-Zeledón Amanda, Jiménez-Rojas César, Roop Ii R Martin, Comerci Diego J, Barquero-Calvo Elías, Chacón-Díaz Carlos, Caswell Clayton C, Baker Kate S, Chaves-Olarte Esteban, Thomson Nicholas R, Moreno Edgardo, Letesson Jean J, De Bolle Xavier, Guzmán-Verri Caterina
Programa de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional de Costa Rica Heredia, Costa Rica.
Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University Greenville, NC, USA.
Front Microbiol. 2016 Sep 29;7:1557. doi: 10.3389/fmicb.2016.01557. eCollection 2016.
Brucellosis is a bacterial infectious disease affecting a wide range of mammals and a neglected zoonosis caused by species of the genetically homogenous genus . As in most studies on bacterial diseases, research in brucellosis is carried out by using reference strains as canonical models to understand the mechanisms underlying host pathogen interactions. We performed whole genome sequencing analysis of the reference strain 2308 routinely used in our laboratory, including manual curated annotation accessible as an editable version through a link at https://en.wikipedia.org/wiki/Brucella#Genomics. Comparison of this genome with two publically available 2308 genomes showed significant differences, particularly indels related to insertional elements, suggesting variability related to the transposition of these elements within the same strain. Considering the outcome of high resolution genomic techniques in the bacteriology field, the conventional concept of strain definition needs to be revised.
布鲁氏菌病是一种影响多种哺乳动物的细菌性传染病，是由基因同源的布鲁氏菌属物种引起的被忽视的人畜共患病。与大多数关于细菌性疾病的研究一样，布鲁氏菌病的研究是通过使用参考菌株作为典型模型来了解宿主与病原体相互作用的潜在机制。我们对实验室常规使用的参考菌株2308进行了全基因组测序分析，包括通过https://en.wikipedia.org/wiki/Brucella#Genomics上的链接可作为可编辑版本获取的人工编辑注释。将该基因组与两个公开可用的2308基因组进行比较，发现存在显著差异，特别是与插入元件相关的插入缺失，这表明这些元件在同一菌株内的转座存在变异性。考虑到细菌学领域高分辨率基因组技术的成果，菌株定义的传统概念需要修订。
