Szilágyi M, Körmendy B, Suri A, Tuboly S, Nagy G
Arch Exp Veterinarmed. 1989 May;43(3):463-70.
Ten male Holstein-Friesian calves naturally infected by Mycobacterium paratuberculosis were experimentally re-infected orally at an average of 17 days. Monthly measurements were conduced of the following activities, in the period between post infection days 160 and 400: total protein (TPR), albumin (ALB), cholesterol (CHOL), triglycerides (TRIG), Zn and Cu concentrations as well as sorbitol dehydrogenase, lactate dehydrogenase (LDH), alpha-hydroxybutyrate dehydrogenase (alpha-HBDH), gamma-glutamyltransferase, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), alkaline phosphatase and fructose-1,6-diphosphate aldolase (ALD). TPR, ALB, TRIG, and CHOL were reduced by day 400, in conjunction with disorders of digestion and absorption. Increased activities of CK, ALD, LDH, alpha-HBDH, AST and ALT primarily indicated damage to skeletal muscle and/or liver. Serum CK and ALD activities as well as TRIG and TPR concentrations may serve as aids to specific diagnosis of paratuberculosis, particularly in the advanced stage of the disease.
十头自然感染副结核分枝杆菌的雄性荷斯坦 - 弗里生犊牛在平均17天时通过口服方式进行了实验性再感染。在感染后第160天至400天期间，每月对以下指标进行测量：总蛋白（TPR）、白蛋白（ALB）、胆固醇（CHOL）、甘油三酯（TRIG）、锌和铜的浓度，以及山梨醇脱氢酶、乳酸脱氢酶（LDH）、α - 羟丁酸脱氢酶（α - HBDH）、γ - 谷氨酰转移酶、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、肌酸激酶（CK）、碱性磷酸酶和果糖 - 1,6 - 二磷酸醛缩酶（ALD）。到第400天时，TPR、ALB、TRIG和CHOL降低，同时伴有消化和吸收紊乱。CK、ALD、LDH、α - HBDH、AST和ALT活性增加主要表明骨骼肌和/或肝脏受损。血清CK和ALD活性以及TRIG和TPR浓度可辅助副结核病的特异性诊断，尤其是在疾病晚期。
