Körmendy B, Szilágyi M, Tuboly S, Nagy G
Central Veterinary Institute, Budapest, Hungary.
Zentralbl Veterinarmed B. 1990 May;37(3):229-35. doi: 10.1111/j.1439-0450.1990.tb01051.x.
Ten bull-calves were infected with 10(8) viable cells of Mycobacterium paratuberculosis per os. During the 400-day period of observation faecal and blood samples were taken from animals at 30-day intervals. Faecal samples were examined microscopically, blood samples by the CFT, AGID and LST tests. Intradermal allergic tests were carried out at PI (post infection) days 92, 217, 336, using mammalian, avian and johnin PPD. In the period of study, these efficiency indices showed fluctuations characteristic of the given tests. In the period between PI day 160 and 400 fifteen biochemical parameters were measured monthly, TRP, ALP, TRIG and CHOL were reduced by day 400, pointing to disorders of digestion and absorption. Increased activities of CK, ALD, LDH, alpha-HBDH and ALT indicated skeletal muscle and/or liver damage in the first place. Serum CK, ALD activities and TRIG and TRP concentrations may serve as useful complementary values to the specific diagnosis of paratuberculosis, particularly in the advanced stage of the disease.
十头公牛犊经口感染了10⁸个副结核分枝杆菌活细胞。在400天的观察期内，每隔30天采集动物的粪便和血液样本。粪便样本进行显微镜检查，血液样本进行补体结合试验（CFT）、琼脂凝胶免疫扩散试验（AGID）和淋巴细胞刺激试验（LST）。在感染后（PI）第92天、217天、336天进行皮内变态反应试验，使用哺乳动物、禽类和副结核菌素纯蛋白衍生物（PPD）。在研究期间，这些效率指标呈现出给定试验特有的波动。在感染后第160天至400天期间，每月测量十五项生化参数，到第400天时，色氨酸（TRP）、碱性磷酸酶（ALP）、甘油三酯（TRIG）和胆固醇（CHOL）降低，表明消化和吸收出现紊乱。肌酸激酶（CK）、醛缩酶（ALD）、乳酸脱氢酶（LDH）、α-羟丁酸脱氢酶（alpha-HBDH）和谷丙转氨酶（ALT）活性增加首先表明骨骼肌和/或肝脏受损。血清CK、ALD活性以及TRIG和TRP浓度可作为副结核病特异性诊断的有用补充指标，尤其是在疾病晚期。
