Alrabiah Haitham, Al-Majed Abdulrahman, Abounassif Mohammed, Mostafa Gamal A E
Acta Pharm. 2016 Dec 1;66(4):503-514. doi: 10.1515/acph-2016-0042.
The fabrication and development of two polyvinyl chloride (PVC) membrane sensors for assaying phenobarbitone sodium are described. Sensors 1 and 2 were fabricated utilizing β- or γ-cyclodextrin as ionophore in the presence of tridodecylmethylammonium chloride as a membrane additive, and PVC and dioctyl phthalate as plasticizer. The analytical parameters of both sensors were evaluated according to the IUPAC guidelines. The proposed sensors showed rapid, stable anionic response (-59.1 and -62.0 mV per decade) over a relatively wide phenobarbitone concentration range (5.0 × 10-6-1 × 10-2 and 8 × 10-6-1 × 10-2 mol L-1) in the pH range of 9-11. The limit of detection was 3.5 × 10-6 and 7.0 × 10-6 mol L-1 for sensors 1 and 2, respectively. The fabricated sensors showed high selectivity for phenobarbitone over the investigated foreign species. An average recovery of 2.54 μg mL-1 phenobarbitone sodium was 97.4 and 101.1 %, while the mean relative standard deviation was 3.0 and 2.1 %, for sensors 1 and 2, respectively. The results acquired for determination of phenobarbitone in its dosage forms utilizing the proposed sensors are in good agreement with those obtained by the British Pharmacopoeial method.
本文描述了两种用于测定苯巴比妥钠的聚氯乙烯（PVC）膜传感器的制备与开发。传感器1和传感器2的制备方法为：以β-或γ-环糊精为离子载体，氯化三癸基甲基铵为膜添加剂，PVC和邻苯二甲酸二辛酯为增塑剂。根据国际纯粹与应用化学联合会（IUPAC）的指导方针评估了两种传感器的分析参数。所提出的传感器在pH值为9 - 11的相对较宽的苯巴比妥浓度范围（5.0×10⁻⁶ - 1×10⁻²和8×10⁻⁶ - 1×10⁻² mol L⁻¹）内显示出快速、稳定的阴离子响应（每十倍浓度变化-59.1和-62.0 mV）。传感器1和传感器2的检测限分别为3.5×10⁻⁶和7.0×10⁻⁶ mol L⁻¹。所制备的传感器对苯巴比妥钠相对于所研究的外来物种具有高选择性。对于2.54 μg mL⁻¹的苯巴比妥钠，传感器1和传感器2的平均回收率分别为97.4%和101.1%，而平均相对标准偏差分别为3.0%和2.1%。利用所提出的传感器测定苯巴比妥钠剂型中的苯巴比妥所获得的结果与英国药典方法所获得的结果高度一致。
