Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Molecules. 2023 Jun 30;28(13):5144. doi: 10.3390/molecules28135144.
Supramolecular gemifloxacin (GF) sensors have been developed. Supramolecular chemistry is primarily concerned with noncovalent intermolecular and intramolecular interactions, which are far weaker than covalent connections, but they can be exploited to develop sensors with remarkable affinity for a target analyte. In order to determine the dose form of the quinolone antibacterial drug gemifloxacin, the current study's goal is to adapt three polyvinylchloride (PVC) membrane sensors into an electrochemical technique. Three new potentiometric membrane sensors with cylindric form and responsive to gemifloxacin (GF) were developed. The sensors' setup is based on the usage of o-nitrophenyl octyl ether (o-NPOE) as a plasticizer in a PVC matrix, β-cyclodextrin (β-CD) (sensor 1), γ-cyclodextrin (γ-CD) (sensor 2), and 4-tert-butylcalix[8]arene (calixarene) (sensor 3) as an ionophore, potassium tetrakis (4-chlorophenyl) borate (KTpClPB) as an ion additive for determination of GF. The developed method was verified according to IUPAC guidelines. The sensors under examination have good selectivity for GF, according to their selectivity coefficients. The constructed sensors demonstrated a significant response towards to GF over a concentration range of 2.4 × 10, 2.7 × 10, and 2.42 × 10 mol L for sensors 1, 2, and 3, respectively. The sensors showed near-Nernstian cationic response for GF at 55 mV, 56 mV, and 60 mV per decade for sensors 1, 2, and 3, respectively. Good recovery and relative standard deviations during the day and between days are displayed by the sensors. They demonstrated good stability, quick response times, long lives, rapid recovery, and precision while also exhibiting good selectivity for GF in various matrices. To determine GF in bulk and dose form, the developed sensors have been successfully deployed. The sensors were also employed as end-point indicators for titrating GF with sodium tetraphenyl borate.
已开发出超分子 gemifloxacin（GF）传感器。超分子化学主要研究非共价的分子间和分子内相互作用，这些相互作用比共价键弱得多，但可以利用它们来开发对目标分析物具有显著亲和力的传感器。为了确定喹诺酮类抗菌药物 gemifloxacin 的剂型，本研究的目的是将三种聚氯乙烯（PVC）膜传感器改编为电化学技术。开发了三种具有圆柱形状且对 gemifloxacin（GF）有响应的新型 PVC 膜电位传感器。传感器的设置基于使用邻硝基苯辛醚（o-NPOE）作为 PVC 基质中的增塑剂，β-环糊精（β-CD）（传感器 1），γ-环糊精（γ-CD）（传感器 2）和 4-叔丁基杯[8]芳烃（杯芳烃）（传感器 3）作为离子载体，四（对氯苯基）硼酸钾（KTpClPB）作为离子添加剂，用于 GF 的测定。该方法是根据 IUPAC 指南进行验证的。根据选择性系数，研究中的传感器对 GF 具有良好的选择性。所构建的传感器对 GF 表现出明显的响应，传感器 1、2 和 3 的浓度范围分别为 2.4×10、2.7×10 和 2.42×10 mol L。传感器 1、2 和 3 对 GF 的响应分别为 55 mV、56 mV 和 60 mV 每 decade，呈近 Nernst 阳离子响应。传感器在白天和之间均表现出良好的回收率和相对标准偏差。它们表现出良好的稳定性，快速的响应时间，长寿命，快速恢复和精度，同时在各种基质中对 GF 具有良好的选择性。已成功将开发的传感器用于测定 GF 的批量和剂型。这些传感器还被用作用四苯硼酸钠滴定 GF 的终点指示剂。
