薯蓣皂苷通过调节长链非编码RNA HOTAIR的表达水平抑制胃癌生长。
Dioscin inhibits gastric tumor growth through regulating the expression level of lncRNA HOTAIR.
作者信息
Ma Ting, Wang Rui-Ping, Zou Xi
机构信息
The Affiliated Hospital of Nanjing University of Chinese Medicine, Traditional Chinese Medicine of Changzhou Hospital, Changzhou, China.
Nanjing University of Chinese Medicine, Nanjing, China.
出版信息
BMC Complement Altern Med. 2016 Sep 30;16(1):383. doi: 10.1186/s12906-016-1360-1.
BACKGROUND
As a member of non-coding RNAs family, long non-coding RNAs' functions in cancer needs to be further investigated. It has been indicated that the functions of Hox transcript antisense intergenic RNA (lncRNA: HOTAIR) include reprogramming chromatin organization and promoting tumor metastasis such as breast and colorectal tumor. The aim of this study is to investigate the functions of Hox in gastric cancer.
METHODS
In the present study, the expression level of HOTAIR was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), 20 gastric cancer tissues and 20 normal tissues was included. All clinical data were analyzed retrospectively. The CCK-8 and colony formation assay was used to identify if the knockdown of HOTAIR have an influence on gastric cancer cell lines.
RESULTS
Compared with normal tissues, higher expression level of HOTAIR was found in gastric cancer tissues. Dioscin inhibits proliferation of the three gastric cancer cell lines and decrease HOTAIR expression.
CONCLUSIONS
The expression of HOTAIR is up regulated in gastric cancer and gastric cancer cell lines, dioscin inhibits the proliferation of three gastric cancer cell lines and the anti-tumor effect of dioscin may partly depend on the down regulation of HOTAIR.
背景
作为非编码RNA家族的一员，长链非编码RNA在癌症中的功能有待进一步研究。已有研究表明，Hox转录反义基因间RNA（长链非编码RNA：HOTAIR）的功能包括重编程染色质组织以及促进肿瘤转移，如乳腺癌和结直肠癌。本研究旨在探讨Hox在胃癌中的功能。
方法
在本研究中，采用定量逆转录聚合酶链反应（qRT-PCR）检测20例胃癌组织和20例正常组织中HOTAIR的表达水平。所有临床资料均进行回顾性分析。采用CCK-8和集落形成试验来确定HOTAIR的敲低是否对胃癌细胞系有影响。
结果
与正常组织相比，胃癌组织中HOTAIR的表达水平更高。薯蓣皂苷抑制三种胃癌细胞系的增殖并降低HOTAIR的表达。
结论
HOTAIR在胃癌及胃癌细胞系中表达上调，薯蓣皂苷抑制三种胃癌细胞系的增殖，且薯蓣皂苷的抗肿瘤作用可能部分依赖于HOTAIR的下调。
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