Department of Gastroenterology, People's Hospital of Taizhou, 366 Taihu Road, Taizhou 225300, Jiangsu, China.
Department of General Surgery, 2nd Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha, Hunan 410011, China.
Can J Gastroenterol Hepatol. 2019 Jun 9;2019:3076345. doi: 10.1155/2019/3076345. eCollection 2019.
BACKGROUND: Long noncoding RNAs (lncRNAs) are a group of noncoding RNA with the length of more than 200nt. They have been identified as important diagnostic and prognostic molecules for many cancers and play an important role in the development of cancers. However, their clinical value and roles in gastric cancer (GC) remain unclear. METHODS: The expression levels of HOTAIR in 54 GC tissues and their matched adjacent nontumor tissues from GC patients and 24 normal mucosa or those with minimal gastritis as healthy controls were determined by qRT-PCR. The expression levels of HOTAIR in human GC cell lines and a normal gastric epithelium cell line were also assessed by qRT-PCR. The potential relationships between its level in GC tissues and the clinicopathological features were analyzed. Furthermore, a receiver operating characteristic (ROC) curve was constructed. Additionally, the correlation between this lncRNA and overall survival (OS) was analyzed. SiRNA transfection was used to silence the expression of HOTAIR in GC cells. And cell proliferation and cell cycle assays were employed to determine the effect of HOTAIR on GC cell growth. Western blot was performed for the detection of the P53, P21, and Bcl2 proteins. RESULTS: The expression levels of HOTAIR were significantly upregulated in GC tissues and cell lines. Increased HOTAIR was associated with tumor differentiation, lymph node and distant metastasis, and clinical stage. Furthermore, the area under the ROC curve (AUC) was up to 0.8416 (95 % CI=0.7661 to 0.9170, P<0.0001). The sensitivity and specificity were 66.67 and 87.04%, respectively. The correlation between HOTAIR expression and overall survival (OS) was statistically significant. The hazard ratio was 2.681, and 95% CI of ratio was 1.370 to 5.248. In addition, knockdown of HOTAIR can inhibit GC cell growth and affect cell cycle distribution. And knockdown of HOTAIR could enhance the protein levels of P21 and P53. CONCLUSION: The present study demonstrated that HOTAIR was highly expressed in GC tissues and may serve as a potential diagnostic and prognostic biomarker for GC. And HOTAIR promoted GC cell proliferation.
背景：长链非编码 RNA（lncRNA）是一组长度超过 200nt 的非编码 RNA。它们已被鉴定为许多癌症的重要诊断和预后分子，并在癌症的发展中发挥重要作用。然而，它们在胃癌（GC）中的临床价值和作用仍不清楚。
方法：通过 qRT-PCR 测定 54 例 GC 患者的 54 例 GC 组织及其配对的相邻非肿瘤组织、24 例正常黏膜或轻度胃炎的健康对照以及人 GC 细胞系和正常胃上皮细胞系中 HOTAIR 的表达水平。分析其在 GC 组织中的水平与临床病理特征之间的潜在关系。此外，构建了受试者工作特征（ROC）曲线。此外，分析了该 lncRNA 与总生存期（OS）的相关性。用 siRNA 转染沉默 GC 细胞中 HOTAIR 的表达。并进行细胞增殖和细胞周期检测，以确定 HOTAIR 对 GC 细胞生长的影响。采用 Western blot 检测 P53、P21 和 Bcl2 蛋白。
结果：HOTAIR 在 GC 组织和细胞系中的表达水平显著上调。HOTAIR 的增加与肿瘤分化、淋巴结和远处转移以及临床分期有关。此外，ROC 曲线下面积（AUC）高达 0.8416（95%CI=0.7661 至 0.9170，P<0.0001）。灵敏度和特异性分别为 66.67%和 87.04%。HOTAIR 表达与总生存期（OS）的相关性具有统计学意义。风险比为 2.681，比值的 95%CI 为 1.370 至 5.248。此外，下调 HOTAIR 可抑制 GC 细胞生长并影响细胞周期分布。下调 HOTAIR 可增强 P21 和 P53 蛋白水平。
结论：本研究表明 HOTAIR 在 GC 组织中高表达，可能作为 GC 的潜在诊断和预后生物标志物。HOTAIR 促进 GC 细胞增殖。
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